Luminescence lifetime standards for the nanosecond to microsecond range and oxygen quenching of ruthenium(II) complexes
Luminescence lifetime standards for the nanosecond to microsecond range and oxygen quenching of ruthenium(II) complexes
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DOI：
10.1021/ac0712796
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发表时间：
2007-12-15
影响因子：
7.4
通讯作者：
DeGraff, B. A.
中科院分区：
文献类型：
--
作者：
Morris, Kaleem J.;Roach, Michael S.;DeGraff, B. A.
本文提出了一种快速、重复性好的测定发光寿命随温度变化的方法。利用该方法建立了几种钌（II）过渡金属配合物的激发态寿命氧猝灭标准。使用三种不同粘度的溶剂和两种寿命相差很大的金属络合物，可以得到重叠的范围。获得了100 ns至6 μ s的脉冲宽度。衰变是纯单指数，这意味着它们可以可靠地用于相位和脉冲寿命仪器。对于纯单指数衰减，正确操作的相移仪器将给出与时域仪器相同的寿命。通过使用热失活模型和三参数温度相关的氧猝灭常数，寿命温度相关的数据通过简单的六参数方程很好地拟合，该方程涵盖10-50摄氏度的温度范围和从0到1个大气压的氧的氧压，具有优异的精度（约2.5 ° C）。< 1%）。这允许对仪器进行实验室和现场校准。
A rapid and reproducible method for determining the temperature dependence of luminescence lifetimes has been developed. With the use of this method, a set of standards for the excited-state lifetime oxygen quenching of several ruthenium(II) transition metal complexes was established. With the use of three solvents of different viscosities and two metal complexes with widely different lifetimes, an overlapping range of ca. 100 ns to 6 mu s was obtained. The decays are pure single exponentials, which means that they can be used reliably with both phase and pulsed lifetime instruments. For a pure single-exponential decay, a properly operating phase shift instrument will give the same lifetime as a time domain instrument. With the use of a thermal deactivation model and a threeparameter temperature-dependent oxygen quenching constant, the lifetime temperature-dependent data was well fit by a simple six-parameter equation that covers the temperature range of 10-50 degrees C and oxygen pressures from 0 to 1 atm of oxygen with excellent precision (ca. < 1%). This permits both laboratory and field calibration of instruments.