In vivo fiber-optic confocal reflectance microscope with an injection-molded plastic miniature objective lens
In vivo fiber-optic confocal reflectance microscope with an injection-molded plastic miniature objective lens
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DOI：
10.1364/ao.44.001792
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发表时间：
2005-04-01
期刊：
影响因子：
1.9
通讯作者：
Richards-Kortum, R
中科院分区：
文献类型：
--
作者：
Carlson, K;Chidley, M;Richards-Kortum, R
为了将体内光学诊断技术推广到发达国家和发展中国家，光学成像系统必须由廉价的部件构成。我们提出了一种具有成本效益的注塑成型塑料微型物镜透镜的光纤共焦反射显微镜在体内成像的人体组织在近真实的时间。测得的横向分辨率小于2.2 μ m，测得的轴向分辨率为10 μ m。以15帧/秒的速度拍摄的离体宫颈组织活检和体内人嘴唇的共聚焦图像证明了显微镜成像细胞形态和组织结构的能力。(c)2005年，美国光学学会。
For in vivo optical diagnostic technologies to be distributed to the developed and developing worlds, optical imaging systems must be constructed of inexpensive components. We present a fiber-optic confocal reflectance microscope with a cost-effective injection-molded plastic miniature objective lens for in vivo imaging of human tissues in near real time. The measured lateral resolution is less than 2.2 mu m, and the measured axial resolution is 10 mu m. Confocal images of ex vivo cervical tissue biopsies and in vivo human lip taken at 15 frames/s demonstrate the microscope's capability of imaging cell morphology and tissue architecture. (c) 2005 Optical Society of America.