OBSERVATIONS OF FOULING BIOFILM FORMATION

OBSERVATIONS OF FOULING BIOFILM FORMATION
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DOI:
10.1139/m81-143
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发表时间:
1981-01-01
影响因子:
2.8
通讯作者:
COSTERTON, JW
COSTERTON, JW
中科院分区:
生物学4区
文献类型:
--
作者:
MCCOY, WF;BRYERS, JD;COSTERTON, JW

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在完全混合的管式循环反应器中监测污垢生物膜的发展。一个独特的采样系统允许直接(明场,落射荧光和扫描电子显微镜)和间接(增加流体摩擦阻力)的生物膜的观察。低流体速度(138.5厘米/秒)实验有较短的诱导时间和生物膜基质,其中包括牢固粘附的丝状细菌。高流体速度(265.4厘米/秒)的实验有较长的诱导时间与牢固粘附的丝状细菌存在后,细胞外物质的积累。在这两种情况下,丝状细菌成为生物膜的永久部分后,流体摩擦阻力增加。
Fouling biofilm development was monitored in a completely mixed tubular recycle reactor. A unique sampling system allowed direct (brightfield, epifluorescence and scanning electron photomicroscopy) and indirect (increased fluid frictional resistant) observations of biofilms. Low fluid velocity (138.5 cm/s) experiments had shorter induction times and biofilm matrixes which included firmly adherent filamentous bacteria. High fluid velocity (265.4 cm/s) experiments had longer induction times with firmly adherent filamentous bacteria present only after the accumulation of extracellular materials. In both cases the fluid frictional resistance increased after filamentous bacteria became a permanent part of the biofilm.