Half-att site substrates reveal the homology independence and minimal protein requirements for productive synapsis in lambda excisive recombination.
Half-att site substrates reveal the homology independence and minimal protein requirements for productive synapsis in lambda excisive recombination.
复制标题
Half-att 位点底物揭示了 lambda 切除重组中有效突触的同源独立性和最低蛋白质需求。
DOI：
10.1016/0092-8674(89)90881-7
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发表时间：
1989
期刊：
影响因子：
64.5
通讯作者：
Landy,A
中科院分区：
文献类型：
--
作者：
Nunes-Düby,SE;Matsumoto,L;Landy,A
The early events in site-specific excisive recombination were studied with phage λ half-attsites that have no DNA to one side of the strand exchange region; they carry a single core-type integrase binding site and either P or P′ arm flanking DNA. These half-attR and half-attL sites exhibit normal properties for the initial (covalent) top-strand transfer and form stable intermediates independent of later steps in the reaction. With these novel substrates we show that Xis specifically promotes the first strand exchange and thatattL enhances Int cleavage at the top-strand site ofattR. It is also shown that synapsis and initial strand transfers do not require DNA-DNA pairing but are mediated by protein-protein and protein-DNA interactions. These involve the two top-strand Int binding sites (required for the first strand exchange) and, in addition, one of the two bottom-strand sites (C′) responsible for the second strand exchange.
