A role for autoantibodies in enhancement of pro-inflammatory cytokine responses to a self-antigen, thyroid peroxidase
A role for autoantibodies in enhancement of pro-inflammatory cytokine responses to a self-antigen, thyroid peroxidase
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DOI：
10.1016/j.clim.2009.07.014
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发表时间：
2009-11-01
影响因子：
8.6
通讯作者：
Hegedus, Laszlo
中科院分区：
文献类型：
--
作者：
Nielsen, Claus H.;Brix, Thomas H.;Hegedus, Laszlo
甲状腺过氧化物酶抗体（TPOAbs）在自身免疫性甲状腺疾病发病机制中的作用尚不清楚。我们从11例桥本甲状腺炎（HT）患者、10例健康的同卵双胞甲状腺炎患者和12例不熟悉AITD倾向的健康个体中选择具有高浓度tpoab的血清，并将每种血清与正常单核细胞（MNCs）混合。在TPO刺激后，MNCs产生的促炎细胞因子tnf - α、IL-6和ifn - γ以及抗炎细胞因子IL-10与培养物中存在的血清TPOAb含量相关（p=0.0002-0.05）。在含胎牛血清的培养基中添加高含量tpoab的IgG，可增强正常跨国公司tpo诱导的tnf - α、IL-6和ifn - γ的产生，并呈剂量依赖性和Fc - γ受体依赖性（p < 0.0002-0.05）。这些数据表明，tpob可促进吞噬细胞释放促炎细胞因子，并促进t细胞对TPO的反应。(C) 2009爱思唯尔公司所有人。版权。
The role of thyroid peroxidase (TPO) antibodies (TPOAbs) in the pathogenesis of autoimmune thyroid disease is unclear. We selected sera with a high concentration of TPOAbs from eleven patients with Hashimoto's thyroiditis (HT), ten healthy monozygotic co-twins to HT patients, and twelve healthy individuals with no familiar disposition to AITD, and mixed each serum with normal mononuclear cells (MNCs). Following challenge with TPO, the MNCs' production of the pro-inflammatory cytokines TNF-alpha, IL-6 and IFN-gamma, and the anti-inflammatory cytokine IL-10, correlated with the TPOAb content of the serum present in the culture (p=0.0002-0.05). Enrichment of foetal calf serum-containing media with IgG with a high content of TPOAbs enhanced the TPO-elicited production of TNF-alpha, IL-6 and IFN-gamma by normal MNCs in a dose- and Fc gamma-receptor dependent manner (p < 0.0002-0.05). The data indicate that TPO-induced release of pro-inflammatory cytokines from phagocytic cells and T-cell responses to TPO are promoted by TPOAbs. (C) 2009 Elsevier Inc. All. rights reserved.