Differences in valvular and vascular cell responses to strain in osteogenic media
Differences in valvular and vascular cell responses to strain in osteogenic media
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DOI：
10.1016/j.biomaterials.2011.01.030
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发表时间：
2011-04-01
期刊：
影响因子：
14
通讯作者：
Nerem, Robert M.
中科院分区：
文献类型：
--
作者：
Ferdous, Zannatul;Jo, Hanjoong;Nerem, Robert M.
钙化是生物修复和组织工程血管和瓣膜移植失败的主要原因。我们使用含有人主动脉平滑肌细胞（HASMC）和人主动脉瓣间质细胞（HAVIC）的组织工程胶原凝胶作为模型，研究细胞介导的钙化早期标志物的差异。hasmc和HAVICs是从非硬化的人体组织中分离出来的。在常规或成骨培养基中分别添加或不添加10%循环应变（1hz）培养21天后，评估胶原凝胶的DNA含量、胶原蛋白I、基质金属蛋白酶(MMP)-2和糖胺聚糖（GAG）含量。与HAVICs相比，含有HASMCs的胶原凝胶含有更多的I型胶原和GAG。菌株在两种细胞类型中均增加了MMP-2活性，但这一趋势是显著的(p
Calcification is the primary cause of failure of bioprosthetic and tissue-engineered vascular and valvular grafts. We used tissue-engineered collagen gels containing human aortic smooth muscle cells (HASMC) and human aortic valvular interstitial cells (HAVIC) as a model to investigate cell-mediated differences in early markers of calcification. The HASMCs and HAVICs were isolated from non-sclerotic human tissues. After 21 days of culture in either regular or osteogenic media with or without 10% cyclic strain at 1 Hz, the collagen gels were assessed for DNA content, collagen I, matrix metalloproteinase (MMP)-2 and glycosaminoglycan (GAG) content. The collagen gels containing HASMCs contained significantly greater amounts of collagen I and GAG compared to HAVICs. Although strain increased MMP-2 activity for both cell types, this trend was significant (p