Regulation of the ASC expression in response to LPS stimulation is related to IL-8 secretion in the human intestinal mucosa
Regulation of the ASC expression in response to LPS stimulation is related to IL-8 secretion in the human intestinal mucosa
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DOI：
10.1016/j.bbrc.2006.06.002
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发表时间：
2006-08-04
影响因子：
3.1
通讯作者：
Inohara, Naohiro
中科院分区：
文献类型：
--
作者：
Masumoto, Junya;Kobayashi, Hitoshi;Inohara, Naohiro
据报道，在体外，半胱天冬酶激活接头ASC的表达与IL-8的产生相关，IL-8是粘膜炎症的主要介质。然而，ASC介导的IL-8在原代组织中产生的意义仍然知之甚少。将从手术切除的回肠或结肠分离的原代肠粘膜与几种浓度的LPS孵育或不处理。低剂量LPS（1-10 ng/ml）刺激后2 h，ASC表达上调，并与IL-8的分泌有关，随后下调。相反，ASC表达在用高剂量LPS（1000 ng/ml）处理的粘膜组织中保持在基础水平。有趣的是，在克罗恩病的几个病例的粘膜中，ASC在没有刺激的情况下高度表达，并且IL-8在没有LPS调节的情况下稳定分泌。这些结果表明，ASC表达与IL-8分泌相关，并可能在维持粘膜稳态中发挥重要作用。(c)2006年爱思唯尔公司All rights reserved.
Expression of the caspase-activating adaptor ASC was reported to be associated with the production of IL-8, a primary mediator of mucosal inflammation, in vitro. However, the significance of the ASC-mediated IL-8 production in primary tissues has remained poorly understood. Primary intestinal mucosa isolated from surgically resected ileum or colon was incubated with several concentrations of LPS or left untreated. ASC expression was up-regulated at 2 h after stimulation with low doses of LPS (1-10 ng/ml), and was associated with IL-8 secretion, and then was down-regulated later. In contrast, ASC expression remained at the basal level in mucosal tissue treated with a high dose of LPS (1000 ng/ml). Interestingly, in mucosa from several cases of Crohn's disease, ASC was highly expressed without stimulation, and IL-8 was stably secreted with no regulation by LPS. These findings revealed that ASC expression correlates with IL-8 secretion and may play an important role in maintaining mucosal homeostasis. (c) 2006 Elsevier Inc. All rights reserved.