Behavioral phenotyping enhanced – beyond (environmental) standardization
Behavioral phenotyping enhanced – beyond (environmental) standardization
复制标题
行为表型分析得到增强——超越（环境）标准化
DOI：
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复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
H. Würbel
中科院分区：
文献类型：
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作者：
H. Würbel
基础生物学认为，动物的表型是先天(基因)和后天(环境)复杂而动态相互作用的产物。然而，这将如何转化为实验设计和动物实验的解释，目前还远不清楚。动物实验是以最大限度的有效性(复杂性)模拟真实世界现象和设计可行的研究项目(抽象)之间的折衷。实验动物学的教科书通常更喜欢抽象，而不是复杂性。然而，根据研究领域的不同，抽象可能会严重影响信息获取，而不是实验设计来模拟的真实世界现象。小鼠突变体的行为表型通常涉及特别复杂的生命表现，如学习、记忆或焦虑，这些都受到环境因素的强烈调节。越来越多的证据表明，目前的行为表型鉴定方法往往产生的结果可能是获得这些结果时所特有的，因为没有考虑到行为表型背后的基因-环境关系的交互性质。本文认为，需要对遗传和环境背景进行系统的变异，而不是过度标准化，以控制结果的稳健性，并检测突变与动物的遗传和环境背景之间的生物相关性交互作用。
It is basic biology that the phenotype of an animal is the product of a complex and dynamic interplay between nature (genotype) and nurture (environment). It is far less clear, however, how this might translate into experimental design and the interpretation of animal experiments. Animal experiments are a compromise between modelling real world phenomena with maximal validity (complexity) and designing practicable research projects (abstraction). Textbooks on laboratory animal science generally favour abstraction over complexity. Depending on the area of research, however, abstraction can seriously compromise information gain, with respect to the real world phenomena an experiment is designed to model. Behavioral phenotyping of mouse mutants often deals with particularly complex manifestations of life, such as learning, memory or anxiety, that are strongly modulated by environmental factors. A growing body of evidence indicates that current approaches to behavioral phenotyping might often produce results that are idiosyncratic to the study in which they were obtained, because the interactive nature of genotype‐environment relationships underlying behavioral phenotypes was not taken into account. This paper argues that systematic variation of genetic and environmental backgrounds, instead of excessive standardization, is needed to control the robustness of the results and to detect biologically relevant interactions between the mutation and the genetic and environmental background of the animals.
测试小鼠行为的遗传学。
DOI：
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发表时间：
1999
期刊：
Science (New York, N.Y.)
影响因子：
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作者：
Tordoff,MG;Bachmanov,AA;Friedman,MI;Beauchamp,GK
通讯作者：
Beauchamp,GK
影响因子：
56.9
作者：
Crabbe, JC;Wahlsten, D;Dudek, BC
通讯作者：
Dudek, BC