Supramolecular architecture of photosynthetic membrane in red algae in response to nitrogen starvation
Supramolecular architecture of photosynthetic membrane in red algae in response to nitrogen starvation
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红藻光合膜响应氮饥饿的超分子结构
DOI：
10.1016/j.bbabio.2016.08.005
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发表时间：
2016
影响因子：
4.3
通讯作者：
Yu-Zhong Zhang
中科院分区：
文献类型：
--
作者：
Long-Sheng Zhao;Hai-Nan Su;Kang Li;Bin-Bin Xie;Lu-Ning Liu;Xi-Ying Zhang;Xiu-Lan Chen;Feng Huang;Bai-Cheng Zhou;Yu-Zhong Zhang
氮的可利用性是限制光合生物包括植物和藻类生长的最重要的决定因素之一;然而，对光合膜响应氮胁迫的超分子结构的直接观察仍然缺乏。红藻是一类重要的藻类进化类群，与蓝藻一样，其类囊体膜上也含有藻胆体。PBS不仅具有捕光天线的功能，而且还具有氮储存的功能。本文报道了红藻紫球藻（Porphyridium cruentum）类囊体膜超分子结构在氮饥饿过程中的变化。完整的类囊体膜为圆形囊泡。在氮饥饿过程中，类囊体膜含量减少，PBSs降解。PBSs的大小和密度都被发现减少。PBS的大小减少了不到一半后，20天的氮饥饿，但它们的半球形形态被保留。随着处理时间的延长，类囊体膜上PBSs的密度受到的影响越来越大。再加氮后，类囊体膜上的PBS增加。这项工作报告的第一个直接观察的变化，在超分子结构的类囊体膜光合生物在氮胁迫的反应。
The availability of nitrogen is one of the most important determinants that can limit the growth of photosynthetic organisms including plants and algae; however, direct observations on the supramolecular architecture of photosynthetic membranes in response to nitrogen stress are still lacking. Red algae are an important evolutionary group of algae which contain phycobilisomes (PBSs) on their thylakoid membranes, as do cyanobacteria. PBSs function not only as light-harvesting antennae but also as nitrogen storage. In this report, alterations of the supramolecular architecture of thylakoid membranes from red algaPorphyridium cruentumduring nitrogen starvation were characterized. The morphology of the intact thylakoid membrane was observed to be round vesicles. Thylakoid membranes were reduced in content and PBSs were degraded during nitrogen starvation. The size and density of PBSs were both found to be reduced. PBS size decreased by less than one-half after 20 days of nitrogen starvation, but their hemispherical morphology was retained. The density of PBSs on thylakoid membranes was more seriously affected as time proceeded. Upon re-addition of nitrogen led to increasing of PBSs on thylakoid membranes. This work reports the first direct observation on alterations in the supramolecular architecture of thylakoid membranes from a photosynthetic organism in response to nitrogen stress.