Synexin enhances the aggregation rate but not the fusion rate of liposomes.
Synexin enhances the aggregation rate but not the fusion rate of liposomes.
复制标题
Synexin 提高脂质体的聚集速率，但不提高脂质体的融合速率。
DOI：
10.1021/bi00412a033
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Hong,K
中科院分区：
文献类型：
--
作者：
Meers,P;Bentz,J;Alford,D;Nir,S;Papahadjopoulos,D;Hong,K
材料和方法牛脑磷脂酰丝氨酸（PS）、磷脂酸（PA）（从蛋磷脂酰胆碱中提取）、蛋磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）（从蛋磷脂酰胆碱中反式酯化）购自Avanti Polar Lipids （Birmingham， AL）。N-[三（羟基甲基）甲基]-2-氨基乙烷磺酸（TES）、二吡啶酸（DPA）（99%）和硝基三乙酸（99%）均来自Sigma （St. Louis， MO）。CaCl2（> 99%）、MgCl2（> 99%）和NaCl（> 99%）来自Fisher （Pittsburgh， PA）。8-氨基萘- 1,3,6 -三磺酸酯（ANTS）和对二甲苯（溴化吡啶）（DPX）来自Molecular Probes （Eugene， OR）。TbCly6H20（99.9%）来自Alfa （Danvers， MA）。聚碳酸酯过滤器来自nucleore （Pleasanton， CA）。Sephadex G-75和cnbr活化的Sepharose 4B来自Pharmacia （Piscataway， NJ）。Ultrogel AcA 34来自LKB Instruments （Rockville， MD）。以氯仿/甲醇/NH4OH（65:25: 5）和氯仿/丙酮/甲醇/乙酸/H20（30:40: 10:10: 5）为溶剂，在硅胶H板（Analtech, Newark， DE）上二维薄层色谱法测定磷脂的纯度。所有磷脂的纯度可估计为99%或更高。联联蛋白的纯化。牛肝联蛋白是通过对先前发表的程序进行修改纯化的（Creutz et al., 1978, 1982; Morris et al., 1982）。净化包括三个主要步骤。(1)牛肝是homo -
Materials and MethodsBovine brain phosphatidylserine (PS), phosphatidate (PA)(derived from egg phosphatidylcholine), egg phosphatidylcholine (PC), and phosphatidylethanolamine (PE)(trans-esterified from egg phosphatidylcholine) were purchased from Avanti Polar Lipids (Birmingham, AL). N-[Tris (hydroxy-methyl) methyl]-2-aminoethanesulfonic acid (TES), dipicolinic acid (DPA)(99%), and nitrilotriacetic acid (99%) were obtianed from Sigma (St. Louis, MO). CaCl2 (> 99%), MgCl2 (> 99%), and NaCl (> 99%) were from Fisher (Pittsburgh, PA). 8-Aminonaphthalene-1, 3, 6-trisulfonate (ANTS) and p-xylylenebis (pyridinium bromide)(DPX) were from Molecular Probes (Eugene, OR). TbCly6H20 (99.9%) was obtained from Alfa (Danvers, MA). Polycarbonate filters were from Nucleopore (Pleasanton, CA). Sephadex G-75 and CNBr-activated Sepharose 4B were from Pharmacia (Piscataway, NJ). Ultrogel AcA 34 was from LKB Instruments (Rockville, MD).The purity of phospholipids was assessed by thin-layer chromatography on silica gel H plates (Analtech, Newark, DE) intwo dimensions using the solvents chloroform/methanol/NH4OH (65: 25: 5) and chloroform/acetone/methanol/acetic acid/H20 (30: 40: 10: 10: 5). The purity of all phospholipids could be estimated as 99% or greater. Purification of Synexin. Bovine liver synexin was purified by using modifications of previously published procedures (Creutz et al., 1978, 1982; Morris et al., 1982). The purifi-cation involved three major steps.(1) Bovine liver was hom-