Translation of rat intestinal RNA yields two alkaline phosphatases.
Translation of rat intestinal RNA yields two alkaline phosphatases.
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大鼠肠道 RNA 的翻译产生两种碱性磷酸酶。
DOI：
10.1042/bj2340563
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Alpers,DH
中科院分区：
文献类型：
--
作者：
Sussman,NL;Seetharam,S;Blaufuss,MC;Alpers,DH
翻译后的总大鼠肠道RNA，免疫沉淀使用单特异性抗血清对大鼠肠道碱性磷酸酶产生两种多肽在成年十二指肠和空肠（分子量62和65 kDa）。两条带的免疫沉淀被单一纯化的碱性磷酸酶阻断。在成年回肠和整个小肠的哺乳pupils，只有62 kDa的翻译产品被发现。脂肪喂养后，翻译碱性磷酸酶增加的量成比例的酶活性的增加，以前在血清中看到。一小部分新生的碱性磷酸酶被易位到微粒体囊泡，产生65和69 kDa的肽。衣霉素处理的膜表现出不同的信号肽的每个翻译产物。翻译产物的N-末端测序显示亮氨酸残基在相似的位置，但与成熟蛋白质序列的重叠没有得到证实。在这些数据的基础上，我们提出了两个mRNA编码碱性磷酸酶在大鼠肠道的存在。
After translation of total rat intestinal RNA, immunoprecipitation using monospecific antiserum against rat intestinal alkaline phosphatase yielded two polypeptides in the adult duodenum and jejunum (molecular masses 62 and 65 kDa). Immunoprecipitation of both bands was blocked by a single purified alkaline phosphatase. In the adult ileum and in the entire small intestine of suckling pups, only the 62 kDa translation product was found. After fat feeding, translated alkaline phosphatase increased by an amount proportionate to the increase in enzyme activity previously seen in the serum. A small fraction of nascent alkaline phosphatase was translocated into microsomal vesicles, producing peptides of 65 and 69 kDa. Tunicamycin-treated membranes demonstrated a different signal peptide for each translation product. N-Terminal sequencing of the translation products showed leucine residues at similar positions, but overlap with the mature protein sequence was not demonstrated. On the basis of these data, we propose the presence of two mRNAs encoding alkaline phosphatase in the rat intestine.