Identification of A Ring-Hydroxylating Dioxygenases Capable of Anthracene and Benz[a] anthracene Oxidization from Rhodococcus sp. P14
Identification of A Ring-Hydroxylating Dioxygenases Capable of Anthracene and Benz[a] anthracene Oxidization from Rhodococcus sp. P14
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红球菌属中能够氧化蒽和苯并[a]蒽的 A 环羟基化双加氧酶的鉴定。
DOI：
10.1159/000494384
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Hu Zhong
中科院分区：
文献类型：
--
作者：
Peng Tao;Luo An;Kan Jie;Liang Lei;Huang Tongwang;Hu Zhong
如今，多环芳烃（PAHs）对土壤和海洋沉积物的污染已成为全世界的一个严重问题。红球菌属P14是从原油污染的沉积物中分离出来的，对多种PAHs表现出降解能力。红球菌属的基因组。对 P14 进行测序。通过生物信息学鉴定了编码与 PAH 降解相关的环羟基化双加氧酶 Baa 的基因簇。当P14以蒽、芘、菲或苯并[a]蒽作为单一碳源培养时，baaA基因的表达水平增加。重组蛋白 Baa 在大肠杆菌 BL21 (DE3) 中过表达。对大肠杆菌中重组蛋白Baa的进一步研究表明，它能够氧化蒽和苯并[a]蒽，分别产生9, 10-二羟基蒽和7, 12-二羟基苯[a]蒽作为代谢物。这些结果表明 Baa 在红球菌属 PAH 降解中发挥重要作用。 P14和Baa在污染环境中PAHs的生物修复中具有潜在的应用前景。
Nowadays, contamination of soil and marine sediments by polycyclic aromatic hydrocarbons (PAHs) has become a serious problem all over the world. Rhodococcus sp. P14 was isolated from sediments with crude oil contaminate and showed degradation ability on various PAHs. The genome of Rhodococcus sp. P14 was sequenced. A gene cluster encoding a ring-hydroxylating dioxygenase Baa related to PAH degradation was identified by bioinformatics. The expression level of gene baaA was increased when P14 was cultured with anthracene, pyrene, phenanthrene, or benz [a] anthracene as the single carbon source. The recombinant protein Baa was overexpressed in E. coli BL21 (DE3). Further investigations on the recombinant protein Baa in E. coli demonstrated that it was able to oxidize anthracene and benz [a] anthracene, resulting in 9, 10-dihydroxyanthracene and 7, 12-dihydroxybenz [a] anthracene as metabolites, respectively. These results indicate that Baa plays an important role in PAH degradation in Rhodococcus sp. P14 and Baa has potential application in the bioremediation of PAHs in the contaminated environment.