Thalamic inputs to cytochrome oxidase-rich regions in monkey visual cortex.
Thalamic inputs to cytochrome oxidase-rich regions in monkey visual cortex.
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DOI：
10.1073/pnas.79.19.6098
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发表时间：
1982-10
影响因子：
11.1
通讯作者：
M. Livingstone;D. Hubel
中科院分区：
文献类型：
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作者：
M. Livingstone;D. Hubel
在灵长类动物初级视皮层，细胞色素氧化酶染色显示出规则排列的斑点状结构，最突出的是II和III层，但也存在于V层和VI层。为了更多地了解这些斑点的输入，我们向猕猴和松鼠的外侧膝状体注射[~3H]Pro或辣根过氧化物酶，并在皮质中寻找运输标记。不出所料，LABEL出现在IVa、IVcα、IVc beta和VI层。此外，两种方法都显示了III层深处的一系列泡腾。在切面上可以看到，泡泡与细胞色素斑点精确匹配。这些结果表明，从外侧膝状体到II/III层深处的斑点区域的投射，或者是间接的，或者更可能是直接的。在细胞色素氧化酶染色的18区，我们还观察到了复杂的带型；这些带型与注射[~3H]Pro或辣根过氧化物酶后的带型非常相似，也类似于用垂直或水平条纹刺激后用2-脱氧葡萄糖标记所产生的带型；Pro和POX标记可能代表从枕区到第18区的投影。
In primate primary visual cortex, staining for cytochrome oxidase reveals a regular array of blob-like structures, most prominent in layers II and III but also present in layers V and VI. In an attempt to learn more about the input to these blobs, we injected the lateral geniculate bodies of macaques and squirrel monkeys with [3H]proline or horseradish peroxidase and looked in the cortex for transported label. As expected, label was present in layers IVa, IVc alpha, IVc beta, and VI. In addition, both methods revealed an array of puffs deep in layer III. Seen in tangential sections, the puffs precisely matched the cytochrome blobs. These results indicate a projection from the lateral geniculate body to the blob regions deep in layer II/III, either indirect via layer IV or more likely direct. In area 18 stained for cytochrome oxidase, we also observed complex banding patterns; these were remarkably similar to the pattern found after [3H]proline or horseradish peroxidase injection and were also similar to the pattern produced with 2-deoxyglucose labeling after stimulation with vertical or horizontal stripes; the proline and peroxidase labels probably represent a projection from the pulvinar to area 18.