Cell Heterogeneity Uncovered by Single-Cell RNA Sequencing Offers Potential Therapeutic Targets for Ischemic Stroke.
Cell Heterogeneity Uncovered by Single-Cell RNA Sequencing Offers Potential Therapeutic Targets for Ischemic Stroke.
复制标题
单细胞RNA测序揭示的细胞异质性为缺血性卒中提供了潜在的治疗靶点。
DOI：
10.14336/ad.2022.0212
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发表时间：
2022-10-01
影响因子：
7.4
通讯作者：
Hu, Bo
中科院分区：
文献类型：
--
作者：
Qiu, Min;Zong, Jia-bin;He, Quan-wei;Liu, Yu-xiao;Wan, Yan;Li, Man;Zhou, Yi-fan;Wu, Jie-hong;Hu, Bo
缺血性卒中是一种有害的神经系统疾病，其特征是不可逆的梗死核心被缺血半暗带（脑组织的可挽救区域）包围。由于脑内细胞的异质性，在缺血性卒中期间神经血管单位和外周免疫细胞内不同脑细胞亚群的独特作用仍然难以捉摸。单细胞RNA测序（scRNA-seq）允许以高分辨率无偏地确定细胞异质性并鉴定细胞标记物，从而揭示细胞类型特异性基因表达模式内的主要脑簇以及细胞特异性亚簇及其在缺血性卒中的不同途径中的功能。在这篇综述中，我们总结了不同细胞类型的分化轨迹的变化，并强调了脑细胞中受中风影响的特定途径和基因。本文的综述有望为在单细胞水平上研究缺血性脑卒中的潜在病理机制和新的治疗策略提供新的研究思路和方向。
Ischemic stroke is a detrimental neurological disease characterized by an irreversible infarct core surrounded by an ischemic penumbra, a salvageable region of brain tissue. Unique roles of distinct brain cell subpopulations within the neurovascular unit and peripheral immune cells during ischemic stroke remain elusive due to the heterogeneity of cells in the brain. Single-cell RNA sequencing (scRNA-seq) allows for an unbiased determination of cellular heterogeneity at high-resolution and identification of cell markers, thereby unveiling the principal brain clusters within the cell-type-specific gene expression patterns as well as cell-specific subclusters and their functions in different pathways underlying ischemic stroke. In this review, we have summarized the changes in differentiation trajectories of distinct cell types and highlighted the specific pathways and genes in brain cells that are impacted by stroke. This review is expected to inspire new research and provide directions for investigating the potential pathological mechanisms and novel treatment strategies for ischemic stroke at the level of a single cell.