Experimental infection of Monodelphis domestica with Rickettsia parkeri
Experimental infection of Monodelphis domestica with Rickettsia parkeri
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DOI：
10.1016/j.ttbdis.2019.101366
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发表时间：
2020-05-01
影响因子：
3.2
通讯作者：
da Fonseca, Adivaldo Henrique
中科院分区：
文献类型：
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作者：
Cordeiro, Matheus Dias;Baeta, Bruna de Azevedo;da Fonseca, Adivaldo Henrique
本研究的目的是评估的有袋动物Monodelphis astritica（灰色短尾负鼠）的感染立克次体parkeri和它的作用作为放大器的细菌Amblyomma蜱。十米用磷酸盐缓冲液（PBS）和106个感染了R. parkeri。在7只动物中，接种为肌内接种，3只为腹膜内接种。一只雄性动物（对照）腹腔内接受1 ml用于接种的相同溶剂。腹腔内接种的三只动物用未感染的A.在感染后第2 - 9天（DPI），雕纹虫幼虫和寄生虫死亡。使用17 kDa和ompA引物，通过聚合酶链反应监测第3至第9 DPI的寄生虫血症。还对动物进行了临床评价。在肌内感染的动物中，只有1只在第5 DPI时血液呈阳性。腹膜内感染的3只动物在DPI第2、5、7和9天血液呈阳性。在10个回收的充血蜱池中，6个具有阳性条带。肌肉内感染动物的肾脏、肝脏、心脏和脾脏也呈阳性。受试动物的直肠温度仅在前三个DPI中升高。腹膜内接种的动物表现出虚脱、被毛变硬和体重减轻。研究发现，R. parkeri能够感染M.蜱，该蜱发展为立克次体并引起异种诊断蜱的感染。
This study aimed to evaluate the infection of the marsupial Monodelphis domestica (the gray short-tailed opossum) by Rickettsia parkeri and its role as an amplifier of the bacterium for Amblyomma ticks. Ten M. domestica males were inoculated with phosphate-buffered saline (PBS) and 106 Vero cells infected with R. parkeri. In seven animals, inoculation was intramuscular, and in three intraperitoneal. One male (control) received 1 ml of the same vehicle used for inoculation intraperitoneally. The three animals inoculated intraperitoneally were infested with uninfected A. sculptum larvae and nymphs between the 2nd and 9th day post-infection (DPI). Parasitemia was monitored from the 3rd to 9th DPI by polymerase chain reaction, using primers for 17 kDa and ompA. The animals were also clinically evaluated. Of the animals infected intramuscularly, only one was blood-positive by the 5th DPI. The three animals infected intraperitoneally were blood-positive on the 2nd, 5th, 7th, and 9th DPI. Of the ten pools of recovered engorged ticks, six had positive bands. The kidney, liver, heart, and spleen of an intramuscularly infected animal were also positive. The rectal temperature of the animals tested increased only in the first three DPI. The animals inoculated intraperitoneally showed prostration, bristled hair, and weight loss. The study found that R. parkeri was capable of infecting M. domestica, which developed rickettsemia and caused infection in xenodiagnostic ticks.