Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise
Molecular Characterization of Hsp47 in Grass Carp (Ctenopharyngodon idella) and Its Correlation with Type I Collagen in Response to Fish Aerobic Exercise
复制标题
草鱼 (Ctenopharyngodon idella) Hsp47 的分子特征及其与鱼类有氧运动反应中 I 型胶原的相关性
DOI：
10.3390/fishes6020017
复制
发表时间：
2021-06-01
期刊：
影响因子：
2.3
通讯作者：
Zhang, Xi
中科院分区：
文献类型：
--
作者：
Liang, Xiao;Wan, Ying;Zhang, Xi
热休克蛋白47（Heat shock protein 47，Hsp 47）是一种胶原特异性分子伴侣，在胶原分子成熟过程中起重要作用。本研究克隆了草鱼（Ctenopharyngodon idella）hsp 47及其类似物的cDNA，并对其进行了鉴定。cDNA的长度分别为1212和1218个碱基对，并包括一个开放的阅读框架，编码403和405个氨基酸。根据推导的氨基酸序列进行分子生物学分析，结果表明，所获得的hsp 47和hsp 47-like cDNA序列正确，推测的蛋白质明显聚为硬骨鱼类进化枝。Hsp 47和Hsp 47-like蛋白的一级结构分析和表征与脊椎动物Hsp 47的基本结构和生物学功能相同。基因表达的空间格局显示，热休克蛋白47和热休克蛋白47样是相对普遍存在于不同的组织和高表达的心脏和皮肤。hsp 47和hsp 47样和I型胶原mRNA的表达水平在不同组织中变化相似。草鱼肌肉中I型胶原蛋白含量随水流速度的增加而显著增加。在有氧运动引起的肌肉中hsp 47、hsp 47-like、col 1a 1和col 1a 2基因表达的变化中，I型胶原的变化与hsp 47表达的相关性最强。此外，col 1a 1与hsp 47-like的相关性最高，col 1a 2与hsp 47的相关性最高。提示草鱼Hsp 47和Hsp 47-like与Ⅰ型胶原合成密切相关。本研究首次提示鱼类有氧运动可以提高草鱼肌肉中I型胶原含量和Hsp 47基因表达。
Heat shock protein 47 (Hsp47) is a collagen-specific molecular chaperone that is indispensable for molecular maturation of collagen. In this study, hsp47 and hsp47-like cDNAs were cloned and characterized in grass carp (Ctenopharyngodon idella). The cDNAs were 1212 and 1218 base pairs long, respectively, and included an open reading frame encoding 403 and 405 amino acids. The molecular phylogeny based on the deduced amino acid sequences indicated that the correct sequences of the hsp47 and hsp47-like cDNA were obtained and the deduced proteins clustered distinctly into teleost clades. Primary structure analysis and characterization of Hsp47 and Hsp47-like shared the basic structure and biofunctions of Hsp47 in vertebrates. The spatial pattern of gene expression revealed that hsp47 and hsp47-like were relatively ubiquitous in different tissues and highly expressed in heart and skin. The expression levels of hsp47 and hsp47-like and type I collagen mRNAs varied similarly in different tissues. Type I collagen content increased significantly with the increase of water velocity in the muscle of grass carp in response to aerobic exercise. Among the gene expression changes of hsp47, hsp47-like, col1a1 and col1a2 in muscle that occurred in response to aerobic exercise, the change of type I collagen was most strongly correlated with hsp47 expression. Additionally, col1a1 showed the highest correlation with hsp47-like and col1a2 showed the highest correlation with hsp47. These findings suggest that grass carp Hsp47 and Hsp47-like are closely related to type I collagen synthesis. This study firstly suggests fish aerobic exercise can improve type I collagen content and Hsp47 gene expression in muscle of grass carp.