A nucleoside-sensitive organic cation transporter in opossum kidney cells.
A nucleoside-sensitive organic cation transporter in opossum kidney cells.
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负鼠肾细胞中的核苷敏感有机阳离子转运蛋白。
DOI：
10.1152/ajprenal.1999.276.2.f323
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Nelson,JA
中科院分区：
文献类型：
--
作者：
Chen,R;Pan,BF;Sakurai,M;Nelson,JA
哺乳动物肾脏有机阳离子和阴离子的分泌是一个多效性的、活跃的过程。一些核苷如脱氧腺苷（dAdo）、2-氯脱氧腺苷和叠氮胸苷由人和啮齿动物的肾脏分泌。以前的工作（J. A. Nelson， J. F. Kuttesch, Jr.和B. H. Herbert）。生化药理学（32:2323-2327,1983）表明经典的有机阳离子转运体（OCT）在dAdo类似物2 ' -脱氧结核素的分泌中发挥作用。使用[14C]四乙基溴化铵（[14C]TEA）作为底物，我们测试了几种肾细胞系对核苷敏感的oct。美国负鼠肾近端小管细胞（OK）表达了对西咪替丁敏感和代谢依赖的外排TEA的能力。其他经典的OCT抑制剂和几种核苷也以反映载体结构特异性的方式抑制这些细胞的TEA外排。核苷对OCT的抑制并不是OCT的普遍特征，因为克隆大鼠肾OCT2在非洲爪蟾卵细胞系统中介导的TEA运输不受相同核苷的抑制。总之，OK细胞似乎拥有一个OCT，它也可能通过一种新的载体运输一些核苷。
Renal secretion of organic cations and anions are pleiotropic, active processes in mammals. Some nucleosides such as deoxyadenosine (dAdo), 2-chlorodeoxyadenosine, and azidothymidine are secreted by human and rodent kidneys. Previous work (J. A. Nelson, J. F. Kuttesch, Jr., and B. H. Herbert.Biochemical Pharmacology32: 2323–2327, 1983) indicated a role for the classic organic cation transporter (OCT) in the secretion of the dAdo analog, 2′-deoxytubercidin, by mouse kidney. Using [14C]tetraethylammonium bromide ([14C]TEA) as a substrate, we tested several renal cell lines for a nucleoside-sensitive OCT. American opossum kidney proximal tubule cells (OK) express a cimetidine-sensitive and metabolic-dependent ability to efflux TEA. Other classic OCT inhibitors and several nucleosides also inhibit TEA efflux by these cells in a manner reflecting structural specificity for the carrier. Inhibition of OCT by nucleosides is not a universal feature of OCTs, since TEA transport mediated by cloned rat kidney OCT2 in theXenopus laevisoocyte system was not inhibited by the same nucleosides. In conclusion, OK cells appear to possess an OCT that may also transport some nucleosides by a novel carrier.