Participation of irradiated Anopheles arabiensis males in swarms following field release in Sudan
Participation of irradiated Anopheles arabiensis males in swarms following field release in Sudan
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DOI：
10.1186/1475-2875-13-484
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发表时间：
2014-12-11
期刊：
影响因子：
3
通讯作者：
El Sayed, Badria B.
中科院分区：
文献类型：
--
作者：
Ageep, Tellal B.;Damiens, David;El Sayed, Badria B.
背景：昆虫不育技术（SIT）的成功取决于释放大量不育雄性，这些雄性能够与目标区域内的野生雄性种群竞争交配。不幸的是，殖民化、大规模生产和辐射过程可能会通过基因选择、自然特征的丧失和体细胞损伤而降低不育雄性的竞争力。在这种情况下，使用标记-释放-重新捕获 (MRR) 技术研究了在距离释放地点不同距离处释放的不育阿拉伯按蚊雄性蚊子的生存、分散和参与群体的能力。方法：为了评估它们对群体的参与情况，将经过辐射和标记的实验室饲养的雄性蚊子连续三个释放到距离大群体已知地点 50、100 或 200 m 的地方。夜晚。随后的夜晚，人们从这大群中收集了雄性。三天内共有 8,100 名男性被释放。根据重新捕获的数据计算平均移动距离 (MDT)、每日生存概率和估计种群规模。观察到释放时雄性年龄对这些参数的影响。结果：三天内释放的雄性中有 5% 被重新捕获。两日龄、三日龄和四日龄雄性中，MDT 分别为 118、178 和 170 m，日生存概率分别为 0.95、0.90 和 0.75。根据每次释放后第一天的重新捕获数据，林肯指数估计自然种群中有 32,546 名雄性。被释放到野外的雄性阿拉伯树能够找到并参与现有的群体，甚至可能发起群体。存活概率随着释放时雄性的年龄而降低，但年长雄性的群体参与度和行走距离似乎高于年轻雄性。因此，纳入预释放期可能有利于雄性的竞争力，并增加成年性别鉴定技术（例如血液尖刺）的吸引力。
Background: The success of the sterile insect technique (SIT) depends the release of large numbers of sterile males, which are able to compete for mates with the wild male population within the target area. Unfortunately, the processes of colonisation, mass production and irradiation may reduce the competitiveness of sterile males through genetic selection, loss of natural traits and somatic damage. In this context, the capacity of released sterile Anopheles arabiensis males to survive, disperse and participate in swarms at occurring at varying distances from the release site was studied using mark-release-recapture (MRR) techniques.Methods: In order to assess their participation in swarms, irradiated and marked laboratory-reared male mosquitoes were released 50, 100 or 200 m from the known site of a large swarm on three consecutive nights. Males were collected from this large swarm on subsequent nights. Over the three days a total of 8,100 males were released. Mean distance travelled (MDT), daily probability of survival and estimated population size were calculated from the recapture data. An effect of male age at the time of release on these parameters was observed.Results: Five per cent of the males released over three days were recaptured. In two-, three-and four-day-old males, MDT was 118, 178 and 170 m, and the daily survival probability 0.95, 0.90 and 0.75, respectively. From the recapture data on the first day following each release, the Lincoln index gives an estimation of 32,546 males in the natural population.Discussion: Sterile An. arabiensis males released into the field were able to find and participate in existing swarms, and possibly even initiate swarms. The survival probability decreased with the age of male on release but the swarm participation and the distance travelled by older males seemed higher than for younger males. The inclusion of a pre-release period may thus be beneficial to male competitiveness and increase the attractiveness of adult sexing techniques, such as blood spiking.