Polyadenylylation destabilizes the rpsO mRNA of Escherichia coli.
Polyadenylylation destabilizes the rpsO mRNA of Escherichia coli.
复制标题
聚腺苷酸化会破坏大肠杆菌 rpsO mRNA 的稳定性。
DOI：
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发表时间：
1995
影响因子：
11.1
通讯作者：
P. Régnier
中科院分区：
文献类型：
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作者：
E. Hajnsdorf;F. Braun;J. Haugel;P. Régnier
编码核糖体蛋白S15的rpsO mRNA只有在与其降解有关的三种核糖核酸酶--核糖核酸酶E、多核苷酸磷酸化酶和核糖核酸酶II失活时才部分稳定。在缺乏核糖核酸酶E和3‘到5’外切核糖核酸酶的菌株中，这种mRNA的降解与转录后伸长分子的出现有关。我们报告说，这些细长的mRNAs含有Poly(A)尾，其中大部分在转录终止的位置融合在3‘-末端发夹的下游。在含有3‘到5’外切核糖核酸酶的菌株中，Poly(A)尾巴较短。如果RNase E失活，多聚(A)聚合酶I(PcnB)的失活可以阻止多聚腺苷酸化，并稳定rpsO mRNA。相反，聚腺苷酸化并不显着改变RNase E介导的rpsO mRNA降解的稳定性。
The rpsO mRNA, encoding ribosomal protein S15, is only partly stabilized when the three ribonucleases implicated in its degradation--RNase E, polynucleotide phosphorylase, and RNase II--are inactivated. In the strain deficient for RNase E and 3'-to-5' exoribonucleases, degradation of this mRNA is correlated with the appearance of posttranscriptionally elongated molecules. We report that these elongated mRNAs harbor poly(A) tails, most of which are fused downstream of the 3'-terminal hairpin at the site where transcription terminates. Poly(A) tails are shorter in strains containing 3'-to-5' exoribonucleases. Inactivation of poly(A) polymerase I (pcnB) prevents polyadenylylation and stabilizes the rpsO mRNA if RNase E is inactive. In contrast polyadenylylation does not significantly modify the stability of rpsO mRNA undergoing RNase E-mediated degradation.