Distribution of HPV16 and 18 intratypic variants in nonnal cytology, intraepithelial lesions, and cervical cancer in a Mexican population
Distribution of HPV16 and 18 intratypic variants in nonnal cytology, intraepithelial lesions, and cervical cancer in a Mexican population
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DOI：
10.1016/j.ygyno.2005.12.002
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发表时间：
2006-08-01
影响因子：
4.7
通讯作者：
Mohar, Alejandro
中科院分区：
文献类型：
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作者：
Lizano, Marcela;De la Cruz-Hernandez, Erick;Mohar, Alejandro
Objective.已经鉴定了HPV 16和18的几种型内变体。这些变异与来自不同地理区域的人群相关，并且在宫颈病变的严重程度之间显示出差异性分布，这很可能是由于不同的致病潜力。本研究的目的是调查墨西哥人群中HPV 16和18的变异分布及其与宫颈病变严重程度和宫颈癌组织学谱系的关系。在412份来自初级卫生保健中心、早期宫颈病变诊所或癌症中心的患者的侵入前和侵入性标本中进行HPV 16和18型检测。使用列联表将HPV变异体的分布与细胞学结果和肿瘤细胞类型相关。使用Fisher精确检验或其RXC表的Fisher-Freeman-Halton扩展检验统计学差异。α值设为P <0.05。在本研究纳入的277名未患癌症的女性中，63.5%（176例）细胞学正常;在其余101名女性中，53.5%为LSIL（54例），46.5%为HSIL（47例）。在总共135例浸润性癌中，78.5%为鳞状细胞癌（106例）; 6.6%为腺癌（9例）; 9.6%为腺鳞癌（ADSC）（13例）; 5.1%为未分化癌（7例）。HPV 16 E和AA-a在浸润前病变和浸润性病变中分布均匀。然而，分离物AA-c仅在宫颈癌中发现。HPV 18 Var-1（E）几乎仅见于浸润性病变，而HPV 18 Var-2（Af）主要见于正常或浸润前病变。在浸润性癌中，这种变异仅见于鳞状细胞癌。我们发现的HPV 16和18变异体在宫颈病变中的差异分布进一步支持了HPV 16和18变异体对宫颈癌发展的不同致病潜力的实验数据。(c)2005年爱思唯尔公司All rights reserved.
Objective. Several intratype variants of HPV16 and 18 have been identified. These variants are associated with populations from different geographic regions, and show a differential distribution among the severity of the cervical lesion, most likely due to different pathogenic potential. The objective of this study was to investigate the variant distribution of HPV16 and 18 in a Mexican population and its association with the severity of the cervical lesion and the histological lineage of cervical cancer.Methods. HPV types 16 and 18 detection was performed in 412 samples of preinvasive and invasive specimens from patients attending a Primary Health-Care Center, an Early Cervical Lesion Clinic, or a Cancer Center. Distribution of HPV variants was correlated with the cytological findings and tumor cell types using contingency tables. Statistical difference was tested with the Fisher's Exact Test or its Fisher-Freeman-Halton extension for RXC tables. Alpha value was set at the P < 0.05.Results. Among the 277 women included in this study without cancer, 63.5% (176 cases) had a normal cytology; from the remaining 101 women, 53.5% were LSIL (54 cases), and 46.5% HSIL (47 cases). From a total of 135 invasive carcinomas, 78.5% were squamous (106 cases); 6.6% adenocarcinoma (9 cases); 9.6% adenosquamous (ADSC) (13 cases); and 5.1% were undifferentiated carcinoma (7 cases). HPV 16 E and AA-a were evenly distributed among preinvasive and invasive lesions. However, the isolate AA-c was exclusively found in cervical cancer. HPV 18 Var-1 (E) was almost exclusively found in invasive lesions, while the HPV 18 Var-2(Af) predominated in normal or preinvasive lesions. In invasive cancer, this variant was found only in squamous tumors.Conclusions. The differential distribution of HPV16 and 18 variants in cervical lesions we found further supports experimental data on the different pathogenic potential of HPV16 and 18 variants for cervical cancer development. (c) 2005 Elsevier Inc. All rights reserved.