Microfluidic Devices for Blood Fractionation
Microfluidic Devices for Blood Fractionation
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DOI：
10.3390/mi2030319
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发表时间：
2011-09-01
期刊：
影响因子：
3.4
通讯作者：
Lim, Chwee Teck
中科院分区：
文献类型：
--
作者：
Hou, Han Wei;Bhagat, Ali Asgar S.;Lim, Chwee Teck
血液是一种复杂的生物流体，由45%的细胞成分悬浮在富含蛋白质的血浆中。这些不同的血液学成分在体内发挥着不同的功能，因此将血液有效地分离成各个成分的能力在临床诊断和生物学研究中都有无数的应用。然而，处理血液并不是微不足道的。在过去的十年里，出现了一系列基于微流控技术的新技术来解决这一紧迫的问题。微流控技术利用其众多优势处理临床血液样本，是这一应用的一种有吸引力的解决方案。本文综述了实现成功分离一种或多种血液成分的各种微流控方法。讨论了从血液细胞成分中分离血浆以及分离感兴趣的血细胞，包括某些稀有细胞的技术。将给出基于包括效率(灵敏度)、纯度(选择性)和吞吐量在内的常见分离指标的比较。最后，我们将深入了解基于血液的分离系统在实现真正的护理点(POC)设备方面所面临的挑战，并提供未来的展望。
Blood, a complex biological fluid, comprises 45% cellular components suspended in protein rich plasma. These different hematologic components perform distinct functions in vivo and thus the ability to efficiently fractionate blood into its individual components has innumerable applications in both clinical diagnosis and biological research. Yet, processing blood is not trivial. In the past decade, a flurry of new microfluidic based technologies has emerged to address this compelling problem. Microfluidics is an attractive solution for this application leveraging its numerous advantages to process clinical blood samples. This paper reviews the various microfluidic approaches realized to successfully fractionate one or more blood components. Techniques to separate plasma from hematologic cellular components as well as isolating blood cells of interest including certain rare cells are discussed. Comparisons based on common separation metrics including efficiency (sensitivity), purity (selectivity), and throughput will be presented. Finally, we will provide insights into the challenges associated with blood-based separation systems towards realizing true point-of-care (POC) devices and provide future perspectives.