Presence of hepatitis B virus in oocytes and embryos: a risk of hepatitis B virus transmission during in vitro fertilization
Presence of hepatitis B virus in oocytes and embryos: a risk of hepatitis B virus transmission during in vitro fertilization
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卵母细胞和胚胎中存在乙型肝炎病毒：体外受精期间存在乙型肝炎病毒传播的风险
DOI：
10.1016/j.fertnstert.2010.12.043
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发表时间：
2011-04-01
影响因子：
6.7
通讯作者：
Zhu, Guijin
中科院分区：
文献类型：
--
作者：
Nie, Rui;Jin, Lei;Zhu, Guijin
目的：探讨慢性乙肝病毒携带者通过卵母细胞和胚胎传播乙肝病毒的危险性。设计：实验室研究。地点：某大学附属医院研究室。患者(S)：31对乙肝表面抗原阴性男性和女性夫妇，41对女性和男性乙肝表面抗原阳性夫妇，39对血清阴性夫妇。干预(S)：无。主要结果衡量(S)：卵细胞和胚胎中的乙肝病毒核酸分析，结果：18对男性HBs Ag阳性/女性HBs Ag阴性夫妇中有3对(84枚胚胎中13枚)和14对男性HBs Ag阴性/女性夫妇中3对(71枚卵母细胞和胚胎中15枚)检出了HBVDNA。在13对男性HBs Ag阳性/女性HBs Ag阴性夫妇中有9对(52枚胚胎中39枚)和17对男性HBs Ag阴性/女性HBs Ag阳性夫妇中8对(63枚卵母细胞和胚胎中30枚)检测到了乙肝病毒RNA。在10对男性HBs Ag阴性/女性HBs Ag阳性夫妇中有6对(13/20个卵母细胞和胚胎中)检测到存在于卵母细胞和胚胎的细胞核和细胞质中的HBs Ag。在39对血清阴性夫妇的135个卵母细胞和胚胎中未检出HBVDNA、HBVRNA和HBSAg。结论：在卵子和胚胎中检出HBVDNA，提示HBVDNA和HBVRNA可能通过生殖系垂直传播。(Fertil Steril(R)2011；95：1667-71。(C)2011年，由美国生殖医学会提供。)
Objective: To define the risk of hepatitis B virus (HBV) transmission through oocytes and embryos from chronic HBV carriers.Design: Laboratory-based study.Setting: Research laboratory in a university hospital.Patient(s): Thirty-one couples with hepatitis B surface antigen (HBsAg)-negative women and HBsAg-positive men, 41 couples with HBsAg-positive women and HBsAg-negative men, and 39 seronegative couples.Intervention(s): None.Main Outcome Measure(s): Hepatitis B virus DNA and RNA analyses in oocytes and embryos, and the location of virus particles containing HBsAgs.Result(s): Hepatitis B virus DNA was detected in 3 of 18 male HBsAg-positive/female HBsAg-negative couples (and in 13 of 84 embryos) and 3 of 14 male HBsAg-negative/female HBsAg-positive couples (and in 15 of 71 oocytes and embryos). Hepatitis B virus RNA was detected in 9 of 13 male HBsAg-positive/female HBsAg-negative couples (and in 39 of 52 embryos) and 8 of 17 male HBsAg-negative/female HBsAg-positive couples (and in 30 of 63 oocytes and embryos). The HBsAg, which is present in the nuclei and cytoplasm of oocytes and embryos, was detected in 6 of 10 male HBsAg-negative/female HBsAg-positive couples (and in 13 of 20 oocytes and embryos). Hepatitis B virus DNA, HBV RNA, and HBsAg were not found in 135 oocytes and embryos from 39 seronegative couples.Conclusion(s): The presence of HBV in oocytes and embryos suggests the possibility of vertical transmission of HBV via the germ line. (Fertil Steril (R) 2011;95:1667-71. (C)2011 by American Society for Reproductive Medicine.)