RNA editing in nascent RNA affects pre-mRNA splicing.
RNA editing in nascent RNA affects pre-mRNA splicing.
复制标题
DOI：
10.1101/gr.231209.117
复制
发表时间：
2018-06
期刊：
影响因子：
7
通讯作者：
Xiao X
中科院分区：
文献类型：
--
作者：
Hsiao YE;Bahn JH;Yang Y;Lin X;Tran S;Yang EW;Quinones-Valdez G;Xiao X
在真核生物中，新生RNA转录物经历一系列复杂的RNA加工步骤以实现mRNA成熟。RNA编辑和选择性剪接是两个主要的RNA加工步骤，可以对最终的基因产物引入显著的修饰。通过孤立地处理这些过程，最近的研究在理解其全球RNA靶点和调控途径方面取得了实质性进展。然而，RNA加工的各个步骤之间的相互作用，基因调控的一个重要方面，仍然知之甚少。通过对不同亚细胞组分的RNA进行测序，我们研究了腺苷到肌苷（A-to-I）RNA编辑的时间及其对可变剪接的影响。我们观察到在多聚腺苷酸化之前，>95%的A至I RNA编辑事件发生在染色质相关RNA中。我们报告了3′受体序列中约500个编辑位点，这些位点可以改变相关外显子的剪接。这些外显子在进化过程中高度保守，并存在于具有重要细胞功能的基因中。此外，我们确定了第二类外显子，其剪接可能由RNA编辑机制识别的RNA二级结构调节。实验验证支持的全基因组分析揭示了RNA编辑和剪接之间的显着相互作用，并扩展了功能性RNA编辑位点的库。
In eukaryotes, nascent RNA transcripts undergo an intricate series of RNA processing steps to achieve mRNA maturation. RNA editing and alternative splicing are two major RNA processing steps that can introduce significant modifications to the final gene products. By tackling these processes in isolation, recent studies have enabled substantial progress in understanding their global RNA targets and regulatory pathways. However, the interplay between individual steps of RNA processing, an essential aspect of gene regulation, remains poorly understood. By sequencing the RNA of different subcellular fractions, we examined the timing of adenosine-to-inosine (A-to-I) RNA editing and its impact on alternative splicing. We observed that >95% A-to-I RNA editing events occurred in the chromatin-associated RNA prior to polyadenylation. We report about 500 editing sites in the 3′ acceptor sequences that can alter splicing of the associated exons. These exons are highly conserved during evolution and reside in genes with important cellular function. Furthermore, we identified a second class of exons whose splicing is likely modulated by RNA secondary structures that are recognized by the RNA editing machinery. The genome-wide analyses, supported by experimental validations, revealed remarkable interplay between RNA editing and splicing and expanded the repertoire of functional RNA editing sites.
登录
查看更多内容
ADAR1结合的基因组分析及其参与多个RNA处理途径。
影响因子：
16.6
作者：
Bahn, Jae Hoon;Ahn, Jaegyoon;Lin, Xianzhi;Zhang, Qing;Lee, Jae-Hyung;Civelek, Mete;Xiao, Xinshu
通讯作者：
Xiao, Xinshu
影响因子：
64.5
作者：
Bhatt DM;Pandya-Jones A;Tong AJ;Barozzi I;Lissner MM;Natoli G;Black DL;Smale ST
通讯作者：
Smale ST
通过在正常和失败的鼠心中进行RNA测序分析转录组复杂性。
影响因子：
20.1
作者：
Lee JH;Gao C;Peng G;Greer C;Ren S;Wang Y;Xiao X
通讯作者：
Xiao X
影响因子：
7
作者：
Harrow J;Frankish A;Gonzalez JM;Tapanari E;Diekhans M;Kokocinski F;Aken BL;Barrell D;Zadissa A;Searle S;Barnes I;Bignell A;Boychenko V;Hunt T;Kay M;Mukherjee G;Rajan J;Despacio-Reyes G;Saunders G;Steward C;Harte R;Lin M;Howald C;Tanzer A;Derrien T;Chrast J;Walters N;Balasubramanian S;Pei B;Tress M;Rodriguez JM;Ezkurdia I;van Baren J;Brent M;Haussler D;Kellis M;Valencia A;Reymond A;Gerstein M;Guigó R;Hubbard TJ
通讯作者：
Hubbard TJ
影响因子：
48
作者：
Katz, Yarden;Wang, Eric T.;Airoldi, Edoardo M.;Burge, Christopher B.
通讯作者：
Burge, Christopher B.