Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats
Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats
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DOI：
10.1371/journal.pone.0215113
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发表时间：
2019-03
期刊：
影响因子：
3.7
通讯作者：
A. Watanabe;Takuro Yamaguchi;K. Murota;Tadaaki Ishii;J. Terao;S. Okada;N. Tanaka;Shinya Kimata;Akira Abe;Tomonori Suzuki;M. Uchino;Y. Niimura
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作者：
A. Watanabe;Takuro Yamaguchi;K. Murota;Tadaaki Ishii;J. Terao;S. Okada;N. Tanaka;Shinya Kimata;Akira Abe;Tomonori Suzuki;M. Uchino;Y. Niimura
加强过氧化氢消除活动在肠道和结肠应防止相关疾病。我们以前分离的乳酸菌，戊糖片球菌Be 1，有利于2-电子还原过氧化氢水。在这项研究中，我们成功地分离出一种替代乳酸菌，植物乳杆菌P1-2，可以有效地减少环境中的烷基氢过氧化物和脂肪酸氢过氧化物，其相应的羟基衍生物，通过2-电子还原。每种菌株表现出广泛的浓度范围内的环境还原活性为每种氢过氧化物。鉴于此，将两种乳酸菌口服给氧敏感性短寿命线虫突变体，这导致其寿命显著延长。这一观察结果表明，戊糖拟青霉Be 1和L. P1-2抑制体内氧化应激。为了确定参与这种反应的特定器官，我们在大鼠中进行了类似的实验，涉及铁超载引起的脂质过氧化反应。我们观察到只有L. plantarum P1-2抑制氧化应激大鼠结肠粘膜脂质过氧化反应。
Reinforcement of hydroperoxide-eliminating activity in the intestines and colon should prevent associated diseases. We previously isolated a lactic acid bacterium, Pediococcus pentosaceus Be1, that facilitates a 2-electron reduction of hydrogen peroxide to water. In this study, we successfully isolated an alternative lactic acid bacterium, Lactobacillus plantarum P1-2, that can efficiently reduce environmental alkyl hydroperoxides and fatty acid hydroperoxides to their corresponding hydroxy derivatives through a 2-electron reduction. Each strain exhibited a wide concentration range with regard to the environmental reducing activity for each hydroperoxide. Given this, the two lactic acid bacteria were orally administered to the oxygen-sensitive short-lived nematode mutant, and this resulted in a significant expansion of its lifespan. This observation suggests that P. pentosaceus Be1 and L. plantarum P1-2 inhibit internal oxidative stress. To determine the specific organs involved in this response, we performed a similar experiment in rats, involving induced lipid peroxidation by iron-overloading. We observed that only L. plantarum P1-2 inhibited colonic mucosa lipid peroxidation in rats with induced oxidative stress.