Allobaculum stercoricanis gen. nov., sp nov., isolated from canin feces
Allobaculum stercoricanis gen. nov., sp nov., isolated from canin feces
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DOI：
10.1016/j.anaerobe.2004.06.004
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发表时间：
2004-10-01
期刊：
影响因子：
2.3
通讯作者：
Collins, MD
中科院分区：
文献类型：
--
作者：
Greetham, HL;Gibson, GR;Collins, MD
对从狗粪便中分离的一种未知的厌氧、过氧化氢酶阴性、不形成孢子的杆状细菌进行了形态学、生物化学和分子遗传学研究。根据细胞形态学和生化试验，该未知细菌初步鉴定为真杆菌属。然而，16S rRNA基因测序研究表明，它与真杆菌属的模式种Eubacterium limosum在遗传学上相距甚远。在系统发生学上，未知物种形成了一个迄今为止未知的亚系，接近一个生物群的基部（命名为rRNA簇XVI），包括无害梭菌、多形性链球菌和一些真杆菌属物种。基于表型和系统发育的标准，建议将该未知细菌分类为一个新的属和种，Allobaculum stercoricanis。使用设计用于鉴定Allobacultan stercoricanis的特异性rRNA靶向探针，原位杂交显示这种新物种代表犬粪便中的重要微生物，占DAPI染色总细胞的0.1%至3.7%（21只犬粪便样品）。立体变斑菌的模式菌株为DSM 13633（T）= CCUG 45212（T）。(C)2004爱思唯尔有限公司保留所有权利。
Morphological, biochemical, and molecular genetic studies were performed on an unknown anaerobic, catalase-negative, nonspore-forming, rod-shaped bacterium isolated from dog feces. The unknown bacterium was tentatively identified as a Eubacterium species, based on cellular morphological and biochemical tests. 16S rRNA gene sequencing studies, however, revealed that it was phylogenetically distant from Eubacterium limosum, the type species of the genus Eubacterium. Phylogenetically, the unknown species forms a hitherto unknown sub-line proximal to the base of a cluster of organisms (designated rRNA cluster XVI), which includes Clostridium innocuum, Streptococcus pleomorphus, and some Eubacterium species. Based on both phenotypic and phylogenetic criteria, it is proposed that the unknown bacterium be classified as a new genus and species, Allobaculum stercoricanis. Using a specific rRNA-targeted probe designed to identify Allobacultan stercoricanis, in situ hybridisation showed this novel species represents a significant organism in canine feces comprising between 0.1% and 3.7% of total cells stained with DAPI (21 dog fecal samples). The type strain of Allobaculum stereoricanis is DSM 13633(T) = CCUG 45212(T). (C) 2004 Elsevier Ltd. All rights reserved.