Immunoglobulin-G binding proteins in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus
Immunoglobulin-G binding proteins in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus
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DOI：
10.1017/s0031182000064908
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发表时间：
1995-08
期刊：
影响因子：
2.4
通讯作者：
H. Wang;P. Nuttall
中科院分区：
文献类型：
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作者：
H. Wang;P. Nuttall
摘要 采用豚鼠免疫球蛋白 G 连接琼脂糖柱检测蜱蜱、附扇扇头蜱、花斑钝蜱和六角硬蜱中的免疫球蛋白 G 结合蛋白 (IGBP)。在所有 3 个物种的未喂食蜱的唾液腺提取物 (SGE) 中均发现了 IGBP。通过 SDS-聚丙烯酰胺凝胶电泳测定，IGBP 的大小因物种而异。在 R.appiculatus 中，在未喂食的雌性和雄性蜱收集的血淋巴中检测到 2 个 IGBP 条带，并且在喂食 6 天的雄性和雌性蜱的血淋巴样本中检测到额外的 78 kDa IGBP。尽管来自未喂食的雄性和雌性阑尾蜱的 SGE 的 IGBP 谱没有差异，但在喂食 6 天后，在雄性蜱的 SGE 中检测到 2 个新的 IGBP。讨论了 IGBP 在蜱中的可能功能。
SUMMARY A guinea-pig immunoglobulin-G linked agarose column was used to detect the immunoglobulin-G binding proteins (IGBP) in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus. IGBPs were found in salivary gland extract (SGE) of the unfed ticks of all 3 species. The sizes of the IGBPs, as determined by SDS–polyacrylamide gel electrophoresis, differed between species. In R. appendiculatus 2 IGBP bands were detected in haemolymph collected from unfed females and males, and an additional 78 kDa IGBP was detected in haemolymph samples of male and female ticks that had fed for 6 days. Although there was no difference in the IGBP profile of SGE derived from unfed male and female R. appendiculatus, 2 new IGBPs were detected in the SGE of the male ticks after they had fed for 6 days. The possible function of IGBP in ticks is discussed.