In vivo imaging of hepatic growth hormone signaling.
In vivo imaging of hepatic growth hormone signaling.
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DOI：
10.1210/me.2005-0532
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发表时间：
2006-11
影响因子：
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通讯作者：
S. Frank;Xiangdong Wang;Kai He;N. Yang;P. Fang;R. Rosenfeld;V. Hwa;T. Chaudhuri;L. Deng;K. Zinn
中科院分区：
文献类型：
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作者：
S. Frank;Xiangdong Wang;Kai He;N. Yang;P. Fang;R. Rosenfeld;V. Hwa;T. Chaudhuri;L. Deng;K. Zinn
我们开发了一种无创的、重复的活体肝脏生长激素信号成像系统。GH调节出生后的生长和代谢。它影响许多组织，但对肝脏有主要影响。我们使用裸鼠进行腺病毒介导的信号转导和转录激活因子5依赖的生长激素反应元件的传递，荧光素酶报告检测生长激素信号通路的激活。我们通过非侵入性生物发光成像检测到GH诱导的完整小鼠肝脏GH信号的一系列变化。肝脏荧光素酶信号在注射GH后3h达到高峰，表现出明显的剂量和时间依赖性。GH受体的联合传递显著增强了GH反应，禁食进一步增强了这种效应。我们的成像系统可以在体内对生长激素信号和作用进行详细的分析，并可能成为研究肝脏和其他组织中其他信号通路的范例。
We developed a system to noninvasively and repeatedly image in vivo hepatic GH signaling. GH regulates postnatal growth and metabolism. It affects numerous tissues, but has major effects in liver. We used nude mice for adenoviral-mediated delivery of a signal transducer and activator of transcription 5-dependent GH response element, a luciferase reporter to detect GH signaling pathway activation. We detected by noninvasive bioluminescence imaging GH-induced hepatic GH signaling serially within intact mice. Statistically significant effects of GH dose and time dependence were detected in the liver luciferase signal that peaked 3 h after GH injection. Codelivery of GH receptor significantly enhanced GH response, an effect that was further augmented by fasting. Our imaging system allows detailed in vivo analysis of GH signaling and action and may be a paradigm for studies of additional signaling pathways in liver and other tissues.