Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
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DOI：
10.3791/58771
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发表时间：
2019-01-01
影响因子：
1.2
通讯作者：
Barisic, Ivan
中科院分区：
文献类型：
--
作者：
Ahmadi, Yasaman;Barisic, Ivan
DNA折纸纳米结构具有用于生物和医学应用的巨大潜力。然而，生理流体中的低盐条件和核酸酶诱导自组装DNA纳米结构的变性和降解。在非病毒基因递送中，通过将带负电荷的DNA包裹在阳离子壳中来克服DNA的酶促降解。在此，受基因递送进展的启发，提出了一种简单、一步且稳健的方法，用于通过用壳聚糖和线性聚乙烯亚胺涂覆DNA折纸纳米结构来稳定它们。聚阳离子涂层有效地保护DNA折纸纳米结构在镁耗尽和核酸酶丰富的媒体。该方法还保留了DNA纳米结构的基于酶和适体的功能化的完全可寻址性。
DNA origami nanostructures hold an immense potential to be used for biological and medical applications. However, low-salt conditions and nucleases in physiological fluids induce denaturation and degradation of self-assembled DNA nanostructures. In non-viral gene delivery, enzymatic degradation of DNA is overcome by the encapsulation of the negatively charged DNA in a cationic shell. Herein, inspired by gene delivery advancements, a simple, one-step and robust methodology is presented for the stabilization of DNA origami nanostructures by coating them with chitosan and linear polyethyleneimine. The polycation coating efficiently protects DNA origami nanostructures in Mg-depleted and nuclease-rich media. This method also preserves the full addressability of enzyme- and aptamer-based functionalization of DNA nanostructures.