Changes in fatty acid composition of Neurospora crassa accompany sexual development and ascospore germination.
Changes in fatty acid composition of Neurospora crassa accompany sexual development and ascospore germination.
复制标题
DOI：
10.1099/00221287-144-7-1713
复制
发表时间：
1998-07
期刊：
影响因子：
1.5
通讯作者：
M. Goodrich-Tanrikulu;Kelly Howe;A. Stafford;M. A. Nelson
中科院分区：
文献类型：
--
作者：
M. Goodrich-Tanrikulu;Kelly Howe;A. Stafford;M. A. Nelson
测定了粗糙脉孢菌性发育各阶段的脂肪酸组成。三酰甘油是主要的酰基脂质在文化进行性发育。受精培养物中的三酰甘油的绝对量随时间而变化，与对照（未受精或假受精）培养物相反，其中三酰甘油的量随年龄线性下降。在能够进行性发育的培养物中，α-亚油酸是主要的脂肪酸，占总脂肪酸质量的53%至65%。α-亚麻酸占总脂肪酸的3%或更少，与通常报道的营养培养物的高得多的水平（10-35%）形成鲜明对比。在受精培养物中，还观察到油酸的质量比略高。这种差异是由于发育中的子囊：在发育中的子囊和成熟的子囊孢子中，油酸取代α-亚油酸作为主要的脂肪酸（占总量的45%至50%）。在萌发的子囊孢子，脂肪酸组成接近营养培养物6小时后，通过热激活诱导萌发。这些结果表明，链孢霉有性组织的脂肪酸组成与无性组织的组成有很大不同，脂肪酸组成的广泛变化与有性发育阶段的几个事件相关。
Fatty acid composition was determined during several stages of sexual development in Neurospora crassa. Triacylglycerol was the predominant acyl lipid in cultures undergoing sexual development. The absolute amounts of triacylglycerol in fertilized cultures varied over time, in contrast to control (unfertilized or mock-fertilized) cultures, in which the amount of triacylglycerol decreased linearly with age. In cultures competent to undergo sexual development, alpha-linoleate was the predominant fatty acid, ranging from 53 to 65% of the total fatty acid mass. alpha-Linolenate was 3% or less of the total fatty acid, in marked contrast to the much higher levels (10-35%) typically reported for vegetative cultures. In fertilized cultures, a slightly higher mass ratio of oleate was also observed. This difference was due to the developing asci: in developing asci and mature ascospores, oleate replaced alpha-linoleate as the predominant fatty acid (45 to 50% of the total). In germinating ascospores, the fatty acid composition approached that of vegetative cultures 6 h after inducing germination by heat activation. These results show that the fatty acid composition of sexual tissues of Neurospora differs substantially from the composition of asexual tissues, and that extensive changes in fatty acid composition correlate with several events in the sexual stage of development.