Influence of acute exercise on renalase and its regulatory mechanism
Influence of acute exercise on renalase and its regulatory mechanism
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DOI：
10.1016/j.lfs.2018.07.042
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发表时间：
2018-10
期刊：
影响因子：
6.1
通讯作者：
Katsuyuki Tokinoya;Jun Shiromoto;T. Sugasawa;Y. Yoshida;Kai Aoki;Y. Nakagawa;H. Ohmori;K. Takekoshi
中科院分区：
文献类型：
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作者：
Katsuyuki Tokinoya;Jun Shiromoto;T. Sugasawa;Y. Yoshida;Kai Aoki;Y. Nakagawa;H. Ohmori;K. Takekoshi
目的肾脏和肝脏中肾酶的表达受核因子（NF）-κB、Sp1和缺氧诱导因子（HIF）-1α的调节。肾酶在急性运动中的表达动态及其机制和生理效应尚不清楚。主要方法21只雄性Wistar大鼠，休息15 min后，在跑台上跑30 min。运动组以10 m/min或30 m/min的速度跑30 min，安静组在跑步机上休息。运动后采集骨骼肌、肾脏、心脏、肝脏和血液样本。通过Western blotting检测骨骼肌中肾酶和磷酸化IkB-α和Akt的表达，通过实时荧光定量RT-PCR检测骨骼肌中HIF-1α、Sp1、MuRF-1和MAFbx的表达。NF-κB调节跖肌中肾酶的表达和比目鱼肌中HIF-1α的表达。与安静组相比，30 m/min组足底肌磷酸化Akt明显增加。急性运动后，骨骼肌中的肾酶表达增加，而其他组织中的肾酶表达减少。这种增加可能是对运动诱导的氧化应激的反应。此外，跖肌中NF-κB可能主要调节肾酶的表达，并支持肾酶的细胞保护作用。
AimsRenalase expression in the kidneys and liver is regulated by nuclear factor (NF)-κB, Sp1, and hypoxia-inducible factor (HIF)-1α. The dynamics of renalase expression in acute exercise, and its mechanism and physiological effects are unclear. We evaluated the effect of different exercise intensities on renalase expression and examined its mechanism and physiological effects.Main methods21 male Wistar rats ran for 30 min on a treadmill after resting for 15 min. The sedentary group rested on the treadmill while the exercise group ran for 30 min at 10 or 30 m/min. Skeletal muscles, the kidney, heart, liver, and blood samples were collected after exercise. The expression of renalase and phosphate IkB-α and Akt was measured by western blotting, while HIF-1α, Sp1, MuRF-1, and MAFbx were measured in the skeletal muscle by real-time RT-PCR.Key findingsRenalase expression in skeletal muscles increased after acute exercise, while its expression in the kidneys, heart, and liver decreased. NF-κB regulated renalase expression in the plantaris muscle and that of HIF-1α in the soleus muscle. Phosphate Akt in the plantaris muscle significantly increased in the 30 m/min group compared with that in the sedentary group. MuRF-1 in the plantaris did not change between these groups.SignificanceRenalase expression in skeletal muscles increased after acute exercise but decreased in other tissues. This increase may be a response to exercise-induced oxidative stress. Furthermore, NF-κB in the plantaris muscle may mainly regulate renalase expression, and support a relationship with the cell protective effects of renalase.