Adipocytic proportion of bone marrow is inversely related to bone formation in osteoporosis
Adipocytic proportion of bone marrow is inversely related to bone formation in osteoporosis
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DOI：
10.1136/jcp.55.9.693
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发表时间：
2002-09-01
影响因子：
3.4
通讯作者：
Byers, RJ
中科院分区：
文献类型：
--
作者：
Verma, S;Rajaratnam, JH;Byers, RJ
目的：初步研究表明，在骨质疏松（OP）骨中脂肪细胞组织增加，支持基质细胞从成骨细胞向脂肪细胞谱系分化的体外证据。要调查这一变化的脂肪组织的比例，以造血/基质组织在OP bone.Methods：脂肪细胞的比例，以造血/基质组织（A/H）进行了测量，通过半自动图像分析在髂嵴活检127例骨质疏松症患者（84例女性患者，48例男性患者，平均年龄，55岁，范围，5-80）。14例组织形态学数据正常的患者（9例女性; 5例男性;平均年龄48岁;范围21-70）作为对照。结果：OP骨的A/H比高于正常对照（OP平均43.06%v正常平均22.4%，p < 0.001）。多元回归分析显示，98.5%的A/H比值的变异性是年龄和骨形成的几个指标的结果，包括松质骨壁厚度、类骨质体积、松质骨厚度、皮质骨壁厚度、松质骨附着率和骨形成率，以及松质骨分离（均具有显著性p < 0.001）。对A/H比影响最大的是松质骨沉积率、类骨质体积和年龄。结论：松质骨沉积率、类骨质体积和年龄与OP骨中脂肪组织比例的增加有关。其中，松质骨沉积率反映成骨细胞活性，表明骨质疏松症中脂肪组织体积的增加与骨形成减少相关，支持骨质疏松症中基质细胞从成骨细胞向脂肪细胞分化途径的假设转换。
Aims: Preliminary studies have suggested that there is an increase in adipocytic tissue in osteoporotic (OP) bone, supporting in vitro evidence for a switch in differentiation of stromal cells from the osteoblastic to the adipocytic lineage. To investigate this the variation of the ratio of adipose tissue to haemopoietic/stromal tissue in OP bone was measured.Methods: The ratio of adipocytic to haemopoietic/stromal tissue (A/H) was measured by semi-automated image analysis in iliac crest biopsies from 127 patients with osteoporosis (84 female patients, 48 male patients; mean age, 55 years; range, 5-80). Fourteen patients with normal histomorphometric data (nine women; five men; mean age, 48 years; range 21-70) acted as controls.Results: The ratio of A/H was higher in OP bone than in the normal controls (OP mean 43.06% v normal mean 22.4%; p < 0.001). Multiple regression analysis showed that 98.5% of the variability in the A/H ratio was the result of age and several measures of bone formation, including cancellous wall thickness, osteoid volume, cancellous thickness, cortical wall thickness, cancellous apposition rate, and bone formation rate, together with cancellous separation (each significant at p < 0.001). Those with the greatest effect on the A/H ratio (in decreasing order) were cancellous apposition rate, osteoid volume, and age.Conclusions: Cancellous apposition rate, osteoid volume, and age were associated with the increase in the proportion of adipose tissue present in OP bone. Of these, cancellous apposition rate reflects osteoblast activity, indicating that the increase in the volume of adipose tissue in osteoporosis is associated with reduced bone formation, supporting the postulated switch in differentiation of stromal cells from the osteoblastic to the adipocytic pathway in osteoporosis.