Studies on cytochrome-c oxidase, XIV. The amino-acid sequence of subunit I--proteinchemical methods for the analysis of a large hydrophobic membrane protein.
Studies on cytochrome-c oxidase, XIV. The amino-acid sequence of subunit I--proteinchemical methods for the analysis of a large hydrophobic membrane protein.
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细胞色素c氧化酶的研究，XIV。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
G. Buse
中科院分区：
文献类型：
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作者：
S. Hensel;G. Buse
牛心脏细胞色素C氧化酶亚基I的氨基酸序列，以前推导出的线粒体DNA的蛋白质化学方法证实。该蛋白质由514个氨基酸组成，Mr为57，060，包括N-末端甲酰基，已得到阳性鉴定。该研究描述了在3%SDS和/或HPLC中通过BioGel-chromatography纯化疏水多肽的方法，以及通过在残留量SDS存在下通过胰凝乳蛋白酶或溴化氰裂解获得的完整肽图进行序列分析。所述方法可用于大的整合膜蛋白的独立测序或用于获得探针以发现基因并为DNA测序提供必要的互补。结果提出了唯一的蛋白质来源的证据，从细菌到人的细胞色素氧化酶的催化亚基的一个家庭的约20个DNA推导的序列。
The amino-acid sequence of bovine heart cytochrome-c oxidase subunit I, previously deduced from mtDNA was corroborated by proteinchemical methods. The protein consists of 514 amino acids, the Mr is 57,060 including the N-terminal formyl group, which is positively identified. The study describes methods for the purification of the hydrophobic polypeptide by BioGel-chromatography in 3% SDS and/or HPLC and the sequence analysis via complete peptide maps obtained either by chymotryptic or cyanogenbromide cleavage in the presence of residual amounts of SDS. The methods may be used either for a stand alone sequencing of large integral membrane proteins or for obtaining probes to find the gene and provide the necessary complement for DNA sequencing. The results present the only protein-derived evidence for a family of about 20 DNA-deduced sequences of the catalytic subunit of cytochrome oxidases from bacteria to man.