Identification of novel synaptonemal complex components in C. elegans
Identification of novel synaptonemal complex components in C. elegans
复制标题
DOI：
10.1083/jcb.201910043
复制
发表时间：
2020-05-04
影响因子：
7.8
通讯作者：
Kim, Yumi
中科院分区：
文献类型：
--
作者：
Hurlock, Matthew E.;Cavka, Ivana;Kim, Yumi
联会复合体(Synaptonemal Complex，SC)是减数分裂过程中在同源染色体之间形成的三组蛋白支架。尽管SC在不同的真核生物中是稳定的同源配对和交叉重组所必需的，但单个组分如何组装成高度保守的SC结构尚不清楚。在此，我们报道了秀丽线虫中两个新的SC组分SYP-5和SYP-6的生化鉴定。SYP-5和SYP-6相互平行，在突触中扮演多余的角色，这为为什么这些基因躲过了之前的基因筛查提供了解释。超分辨显微镜显示，它们定位在染色体轴之间，以头对头的方式跨越SC的宽度，类似于其他已知的横丝蛋白的取向。利用遗传冗余和结构-功能分析截断SYP-5/6的C-末端，我们提供了证据支持SC在限制和促进交换形成中的作用。
The synaptonemal complex (SC) is a tripartite protein scaffold that forms between homologous chromosomes during meiosis. Although the SC is essential for stable homologue pairing and crossover recombination in diverse eukaryotes, it is unknown how individual components assemble into the highly conserved SC structure. Here we report the biochemical identification of two new SC components, SYP-5 and SYP-6, in Caenorhabditis elegans. SYP-5 and SYP-6 are paralogous to each other and play redundant roles in synapsis, providing an explanation for why these genes have evaded previous genetic screens. Superresolution microscopy reveals that they localize between the chromosome axes and span the width of the SC in a head-to-head manner, similar to the orientation of other known transverse filament proteins. Using genetic redundancy and structure-function analyses to truncate C-terminal tails of SYP-5/6, we provide evidence supporting the role of SC in both limiting and promoting crossover formation.