Regulation of biofilm formation in Zymomonas mobilis to enhance stress tolerance by heterologous expression of pfs and luxS.
Regulation of biofilm formation in Zymomonas mobilis to enhance stress tolerance by heterologous expression of pfs and luxS.
复制标题
通过pfs和luxS的异源表达调节运动发酵单胞菌生物膜形成以增强胁迫耐受性。
DOI：
10.3389/fbioe.2023.1130405
复制
发表时间：
2023
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
Zymomonas mobilis is a potential alternative of Saccharomyces cerevisiae to produce cellulosic ethanol with strengths in cofactor balance, but its lower tolerance to inhibitors in the lignocellulosic hydrolysate restricts the application. Although biofilm can improve bacteria stress tolerance, regulating biofilm formation in Z. mobilis is still a challenge. In this work, we constructed a pathway by heterologous expressing pfs and luxS from Escherichia coli in Z. mobilis to produce AI-2 (autoinducer 2), a universal quorum-sensing signal molecule, to control cell morphology for enhancing stress tolerance. Unexpectedly, the results suggested that neither endogenous AI-2 nor exogenous AI-2 promoted biofilm formation, while heterologous expression of pfs can significantly raise biofilm. Therefore, we proposed that the main factor in assisting biofilm formation was the product accumulated due to heterologous expression of pfs, like methylated DNA. Consequently, ZM4::pfs produced more biofilm, which presented an enhanced tolerance to acetic acid. All these findings provide a novel strategy to improve the stress tolerance of Z. mobilis by enhancing biofilm formation for efficient production of lignocellulosic ethanol and other value-added chemical products.
登录
查看更多内容
影响因子：
6.3
作者：
Franden MA;Pilath HM;Mohagheghi A;Pienkos PT;Zhang M
通讯作者：
Zhang M
影响因子：
5
作者：
Huang, Suzhen;Xue, Tingli;Zhang, Minhua
通讯作者：
Zhang, Minhua
影响因子：
2.7
作者：
Mizan, Md Furkanur Rahaman;Jahid, Iqbal Kabir;Ha, Sang-Do
通讯作者：
Ha, Sang-Do
DOI：
10.1007/s10295-013-1287-1
发表时间：
2013-08-01
影响因子：
3.4
作者：
Jia, Xianghui;Wei, Na;Wang, Haoyong
通讯作者：
Wang, Haoyong
影响因子：
3.8
作者：
Todhanakasem, Tatsaporn;Yodsanga, Supanika;Champreda, Verawat
通讯作者：
Champreda, Verawat