Cryptosporidium parvum infection in orphan lambs on a farm open to the public
Cryptosporidium parvum infection in orphan lambs on a farm open to the public
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DOI：
10.1136/vr.161.1.11
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发表时间：
2007-07-07
影响因子：
2.2
通讯作者：
Marshall, R. N.
中科院分区：
文献类型：
--
作者：
Pritchard, G. C.;Marshall, J. A.;Marshall, R. N.
在一个开放式农场进行了纵向调查，以调查从当地三个羊群获得的孤儿羔羊隐孢子虫种感染的发生。在最初的试点研究，隐孢子虫卵囊检测荧光抗体试验（FAT）的粪便中的两个21岁之间的一个和三个星期来自一个羊群（羊群A）。每周从来自每个羊群的羔羊中收集粪便的合并围栏样本;通过FAT在来自A群羔羊的49个样本中的24个（49.0%）、来自B群羔羊的59个样本中的18个（30.5%）和来自C群羔羊的47个样本中的14个（29.8%）中检测到卵囊。在12周龄以下的羔羊中，每克粪便中检测到1 × 10（3）至2 × 10（6）以上的卵囊计数，A群和B群的羔羊在6周龄时达到峰值，C群的羔羊在4周龄时达到峰值。经分子生物学鉴定，卵囊为微小隐孢子虫。几乎所有的感染都是亚临床的。
A longitudinal survey was undertaken on an open farm to investigate the occurrence of Cryptosporidium species infection in orphan lambs obtained from three local flocks. During an initial pilot study, Cryptosporidium oocysts were detected by a fluorescent antibody test (FAT) in the faeces of two of 21 lambs aged between one and three weeks derived from one flock (flock A). Pooled pen samples of faeces were collected weekly from lambs derived from each flock; oocysts were detected by FAT in 24 (49.0 per cent) of 49 samples from lambs from flock A, 18 (30.5 per cent) of 59 samples from lambs from flock B and 14 (29.8 per cent) of 47 samples from lambs from flock C. Oocyst counts of 1 x 10(3) to more than 2 x 10(6) per gram of faeces Were detected in lambs up to 12 weeks old, with the peak counts occurring at six weeks of age in the lambs from flocks A and B and at four weeks of age in those from flock C. The oocysts were confirmed by molecular analysis as Cryptosporidium parvum. Virtually all the infections were subclinical.