NEW BACTERIAL GENE (GROPC) WHICH AFFECTS LAMBDA-DNA REPLICATION
NEW BACTERIAL GENE (GROPC) WHICH AFFECTS LAMBDA-DNA REPLICATION
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DOI：
10.1007/bf00446910
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
GEORGOPOULOS, CP
中科院分区：
文献类型：
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作者：
GEORGOPOULOS, CP
一种影响大肠杆菌的细菌突变。被称为groPC756的DNA复制被定位在thr和leu细菌基因座之间。大部分的父母. DNA在该宿主中甚至不经历1轮复制。.变种人叫. pi地图上显示的是。P基因能够克服groPC756突变的抑制作用。groPC基因座上的突变在42 ℃干扰细菌生长。C. A.携带groPC+等位基因的转导噬菌体作为噬斑形成剂在groPC 756细菌上分离。溶原化后，它恢复了gro+和耐温表型。
A bacterial [Escherichia coli] mutation affecting .lambda. DNA replication, called groPC756, was mapped between the thr and leu bacterial loci. Most of the parental .lambda. DNA does not undergo even 1 round of replication in this host. .lambda. Mutants called .pi. which map in the .lambda. P gene are able to overcome the inhibitory effect of the groPC756 mutation. The mutation at the groPC locus interferes with bacterial growth at 42.degree. C. A .lambda. transducing phage carrying the groPC+ allele was isolated as a plaque-former on groPC756 bacteria. Upon lysogenization it restores the gro+ and temperature resistant phenotypes.