GC-MS-based metabolic profiling reveals metabolic changes in anaphylaxis animal models
GC-MS-based metabolic profiling reveals metabolic changes in anaphylaxis animal models
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DOI：
10.1007/s00216-012-6129-x
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发表时间：
2012-08-01
影响因子：
4.3
通讯作者：
Liu, Dong-bo
中科院分区：
文献类型：
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作者：
Hu, Xia;Wu, Gong-ping;Liu, Dong-bo
过敏反应的临床定义和适当管理是一项临床挑战，因为临床体征和症状的变异性很大。监测过敏反应的代谢状态可能有助于了解其病理生理过程和诊断。本研究的目的是对过敏反应动物模型进行GC-MS血清代谢谱分析，并寻找潜在的过敏反应生物标志物。将36只豚鼠随机分为卵白蛋白组（n = 12）、牛白蛋白组（n = 12）和对照组（n = 12）。采用酶联免疫吸附试验（ELISA）检测豚鼠血清中IgE水平，采用气相色谱-质谱联用（GC-MS）检测血清中主要代谢变化。用卵白蛋白或牛白蛋白攻毒后，动物出现典型的临床症状。两模型组血清IgE水平均显著高于对照组。观察到三组基于变量的聚类趋势，发现对照组和模型组之间858个代谢组学特征中有9个存在显著差异。在这9个特征中，有6个特征被初步确定为与过敏反应中的能量代谢和信号转导有关的代谢产物。因此，基于GC-MS的代谢谱分析可能是研究过敏反应的有效辅助工具。
Clinical definition and appropriate management of anaphylaxis is a clinical challenge because there is large variability in presenting clinical signs and symptoms. Monitoring of the metabolic status of anaphylaxis may be helpful in understanding its pathophysiological processes and diagnosis. The purpose of this study was to conduct GC-MS serum metabolic profiling of anaphylaxis animal models and search for potential biomarkers of anaphylaxis. Thirty-six guinea pigs were randomly divided into an ovalbumin group (n = 12), a cattle albumin group (n = 12), and a control group (n = 12). The IgE level in the serum of the guinea pigs was evaluated by use of ELISA kits and the major metabolic changes in serum were detected by gas chromatography-mass spectrometry. Typical clinical symptoms appeared after the animals had been challenged with ovalbumin or cattle albumin. The IgE levels in serum of both model groups were significantly higher than those of the control group. Clustering trend of the three groups based on variables was observed and nine out of 858 metabolomic features were found to be significantly different between control group and model groups. Among the nine features, six features were tentatively identified as metabolites related to energy metabolism and signal transduction in anaphylaxis. In conclusion, GC-MS-based metabolic profiling analysis might be an effective auxiliary tool for investigation of anaphylaxis.