Inter- and intraspecific sequence variations of the chloroplast genome in wild and cultivated Raphanus
Inter- and intraspecific sequence variations of the chloroplast genome in wild and cultivated Raphanus
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DOI：
10.1111/j.1439-0523.2008.01558.x
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发表时间：
2009-04-01
期刊：
影响因子：
2
通讯作者：
Ishibashi, A.
中科院分区：
文献类型：
--
作者：
Yamagishi, H.;Terachi, T.;Ishibashi, A.
为了评估萝卜属野生种和栽培种叶绿体基因组的差异，研究了萝卜属植物从trnL（UAA）到psbG的约2kbp的核苷酸序列多态性。raphanistrum和R.大蒜野生种18种，日本野萝卜10种（R. sativus），31株栽培植物用于序列分析。野生和栽培萝卜属植物的叶绿体基因组均存在种内变异。研究的所有三个基因（trnL、trnF和ndhJ）在属内含有核苷酸取代。然而，在基因间区观察到大量的突变。利用检测到的变异，将59个萝卜植株分为11个单倍型，其中7个是野生种所特有的。在单倍型中，有一种与Ogura雄性不育细胞质完全对应。所有栽培萝卜都属于四种类型之一，其中三种也在日本野生萝卜中观察到。聚类分析将单倍型划分为4类，在系统树图中的分布证实了栽培萝卜的多源性。另一方面，在R.萝卜属植物的细胞质遗传多样性是栽培萝卜育种的重要材料。
To assess the differentiation of the chloroplast genome in wild and cultivated species of Raphanus, nucleotide sequence polymorphisms were investigated for approximately 2 kbp ranging from trnL (UAA) to psbG in R. raphanistrum and R. sativus. Eighteen plants of wild species, 10 Japanese wild radish plants (R. sativus), and 31 cultivated plants were used for sequence analysis. Intraspecific variations of the chloroplast genome were present both in wild and cultivated Raphanus. All three genes investigated (trnL, trnF and ndhJ) contained nucleotide substitutions within the genus. Whereas, larger numbers of mutations were observed in the intergenic regions. Using the detected variations, the 59 radish plants were classified into 11 haplotypes, seven of which were unique to wild species. Among the haplotypes, one type corresponded completely with the Ogura male sterile cytoplasm. All the cultivated radishes belonged to one of four types, of which three were also observed in Japanese wild radish. The haplotypes were classified into four groups by cluster analysis, and the distribution in the dendrogram confirmed that cultivated radish has multiple origins. On the other hand, the seven haplotypes uniquely observed in R. raphanistrum were considered as useful materials to provide genetic diversity of cytoplasm for breeding of cultivated radishes.