Ultrasensitive lateral-flow assays via plasmonically active antibody-conjugated fluorescent nanoparticles
Ultrasensitive lateral-flow assays via plasmonically active antibody-conjugated fluorescent nanoparticles
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DOI：
10.1038/s41551-022-01001-1
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发表时间：
2023-02-02
影响因子：
28.1
通讯作者：
Singamaneni, Srikanth
中科院分区：
文献类型：
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作者：
Gupta, Rohit;Gupta, Prashant;Singamaneni, Srikanth
侧向层析检测 (LFA) 快速且廉价，但其灵敏度比实验室检测低近 1,000 倍。在这里，我们展示了等离激元活性抗体偶联的荧光金纳米棒可以使传统的 LFA 变得超灵敏。从样本到答案的时间在 20 分钟内，通过标准台式荧光扫描仪读出的等离子体增强 LFA 的动态范围和检测限比 4 小时金标准酶联免疫吸附测定提高了约 30 倍，并且对严重急性呼吸综合征冠状病毒 2 患者血浆中的抗体和鼻咽拭子中的抗原实现了 95% 的临床灵敏度和 100% 的特异性（SARS-CoV-2）。通过廉价的便携式扫描仪也可以实现检测性能的类似改进，正如我们在检测人血清样本中的白细胞介素 6 和鼻咽样本中的 SARS-CoV-2 核衣壳蛋白时所展示的那样。等离激元增强的 LFA 在灵敏度、速度、动态范围、易用性和成本方面优于标准实验室测试，并且可能在现场诊断中提供优势。通过使用等离激元活性荧光金纳米棒作为抗体偶联标记，可以将传统侧流测定的灵敏度提高几个数量级。
Lateral-flow assays (LFAs) are rapid and inexpensive, yet they are nearly 1,000-fold less sensitive than laboratory-based tests. Here we show that plasmonically active antibody-conjugated fluorescent gold nanorods can make conventional LFAs ultrasensitive. With sample-to-answer times within 20 min, plasmonically enhanced LFAs read out via a standard benchtop fluorescence scanner attained about 30-fold improvements in dynamic range and in detection limits over 4-h-long gold-standard enzyme-linked immunosorbent assays, and achieved 95% clinical sensitivity and 100% specificity for antibodies in plasma and for antigens in nasopharyngeal swabs from individuals with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Comparable improvements in the assay's performance can also be achieved via an inexpensive portable scanner, as we show for the detection of interleukin-6 in human serum samples and of the nucleocapsid protein of SARS-CoV-2 in nasopharyngeal samples. Plasmonically enhanced LFAs outperform standard laboratory tests in sensitivity, speed, dynamic range, ease of use and cost, and may provide advantages in point-of-care diagnostics.The sensitivity of conventional lateral-flow assays can be improved by orders of magnitude by using plasmonically active fluorescent gold nanorods as antibody-conjugated labels.