Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings
Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings
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作者:
Yanlin Liu;Yan Xiong
Target of rapamycin (TOR) is a central regulator in nutrient signaling. However,.the innate capacity of autotrophic plants to produce carbon-related nutrients.and nitrogen-related nutrients makes studying the TOR pathway difficult. Here,.we describe a protocol for a liquid culture system for efficient depletion of the.endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous car-.bon/nitrogen can be supplied to dissect the TOR pathway..For complete details on the use and execution of this protocol, please refer to.Xiong et al. (2013) and Liu et al. (2021)..