Hyaluronic acid hydrogel modified with nogo-66 receptor antibody and poly-L-lysine to promote axon regrowth after spinal cord injury.
Hyaluronic acid hydrogel modified with nogo-66 receptor antibody and poly-L-lysine to promote axon regrowth after spinal cord injury.
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DOI：
10.1002/jbm.b.31689
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发表时间：
2010-10
期刊：
影响因子：
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通讯作者：
Yue-teng Wei;Yu He;Changlei Xu;Ying Wang;Bingfang Liu;Xiu-mei Wang;Xiao-dan Sun;F. Cui;
中科院分区：
文献类型：
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作者：
Yue-teng Wei;Yu He;Changlei Xu;Ying Wang;Bingfang Liu;Xiu-mei Wang;Xiao-dan Sun;F. Cui;
用于中枢神经系统损伤的生物材料不仅需要与特定的细胞粘附相互作用，还需要与特定的生长因子受体相互作用以促进神经再生。在本研究中，用聚l -赖氨酸（PLL）和nogo-66受体抗体（antiNgR） （HA-PLL/antiNgR）修饰的透明质酸（HA）为基础的水凝胶在脊髓外侧半切后给予大鼠。植入后8周，HA-PLL/抗ingr水凝胶中出现抗神经丝阳性轴突延伸，与HA-PLL或空白对照组比较差异有统计学意义。电镜观察发现，HA-PLL/anti - ingr水凝胶比HA-PLL水凝胶有更多的髓鞘细胞和正常轴突。血凝素水凝胶在体内移植8周后仍能检测到抗ingr。这些特性有助于HA-PLL/抗ingr水凝胶成为修复脊髓损伤的有前景的支架材料。然而，两种改性水凝胶（HA-PLL/antiNgR和HA-PLL）在支持血管生成和抑制胶质瘢痕形成方面均表现出显著的优势。
The biomaterials used for central nervous system injury require not only interacting with specific cell adhesion but also specific growth factor receptors to promote nerve regeneration. In this study, hyaluronic acid (HA)-based hydrogels modified with poly-L-lysine (PLL) and nogo-66 receptor antibody (antiNgR) (HA-PLL/antiNgR) were administered to rats after lateral hemisection of the spinal cord. Anti-neurofilament positive axons were found to extend into the HA-PLL/antiNgR hydrogel at 8 weeks after implantation, which shows significant difference compared with HA-PLL or blank control group. Electron micrographs of implanted hydrogels showed that there were more cells and normal axons with myelin in the HA-PLL/antiNgR implant than that of HA-PLL hydrogel. The antiNgR grafted on HA hydrogels could be detected for 8 weeks after transplantation in vivo. All of these properties may facilitate HA-PLL/antiNgR hydrogels to become a promising scaffold for repairing spinal cord injury. Nevertheless, both two kinds of modified hydrogels (HA-PLL/antiNgR and HA-PLL) showed remarkable advantages in supporting angiogenesis, and simultaneously inhibiting the formation of glial scar.