Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX.
Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX.
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DOI：
10.1107/s1744309110011851
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发表时间：
2010-05-01
期刊：
影响因子：
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通讯作者：
Howell PL
中科院分区：
文献类型：
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作者：
Weadge JT;Yip PP;Robinson H;Arnett K;Tipton PA;Howell PL
AlgX是假单胞菌属和棕色固氮菌中产生胞外多糖藻酸盐所需的周质蛋白。AlgX已被过度表达和纯化，衍射质量的晶体已使用迭代播种和悬滴气相扩散法生长。晶体呈平板状生长，晶胞参数a = 46.4，B = 120.6，c = 86.9 °，β = 95.7°。晶体具有空间群P21的对称性，衍射的最小d间距为2.1 μ m。根据马修斯系数（VM = 2.25 3 Da−1），估计在不对称单元中存在两个分子。
AlgX is a periplasmic protein required for the production of the exopolysaccharide alginate in Pseudomonas sp. and Azotobacter vinelandii. AlgX has been overexpressed and purified and diffraction-quality crystals have been grown using iterative seeding and the hanging-drop vapor-diffusion method. The crystals grew as flat plates with unit-cell parameters a = 46.4, b = 120.6, c = 86.9 Å, β = 95.7°. The crystals exhibited the symmetry of space group P21 and diffracted to a minimum d-spacing of 2.1 Å. On the basis of the Matthews coefficient (VM = 2.25 Å3 Da−1), two molecules were estimated to be present in the asymmetric unit.