Comparative proteomic analyses using iTRAQ-labeling provides insights into fiber diversity in sheep and goats
Comparative proteomic analyses using iTRAQ-labeling provides insights into fiber diversity in sheep and goats
复制标题
使用 iTRAQ 标记进行比较蛋白质组学分析可深入了解绵羊和山羊的纤维多样性
DOI：
10.1016/j.jprot.2017.10.008
复制
发表时间：
2018-02-10
影响因子：
3.3
通讯作者：
Wang, Xiaolong
中科院分区：
文献类型：
--
作者：
Li, Yan;Zhou, Guangxian;Wang, Xiaolong
羊毛和头发纤维的结构成分，如角蛋白相关蛋白（KAP），已经得到了很好的描述，但纤维直径的遗传决定因素在很大程度上是未知的。在这里，我们使用了基于iTRAQ的蛋白质组学方法来研究绵羊和山羊的18个样本在不同纤维范围内的蛋白质丰度差异。我们鉴定了具有不同丰度的蛋白质，并与纤维特征的变化相关。不同丰度的蛋白质主要是角蛋白或角蛋白相关蛋白（KRTAP 11 -1、KRT 6A、KRT 38），或与毛发生长（DSC 2、DSG 3、EEF 2、CALML 5、TCHH、SELENBP 1）和脂肪酸合成（FABP 4、FABP 5）相关。RNA-seq进一步证实了DSC 2基因在确定山羊纤维中的羊毛表型中的功能重要性。这种对主要纤维生产动物纤维的全面分析首次提供了参与纤维形成的候选蛋白质列表。生物学意义：蛋白质是动物源性毛发纤维的基础，但绵羊和山羊中赋予纤维结构和特征的蛋白质在很大程度上是难以捉摸的。通过iTRAQ方法检查来自绵羊和山羊的代表9种纤维类型的27个纤维样品，我们显示了对毛发结构组分或与毛发生长和脂肪酸合成相关的基因重要的差异丰富蛋白质的列表。RNA-seq进一步验证了DSC 2基因是山羊中羊毛/直发表型的关键。
The structural component of wool and hair fibers, such as keratin-associated proteins (KAPs), has been well described, but the genetic determinants of fiber diameter are largely unknown. Here, we have used an iTRAQ-based proteomic approach to investigate differences in protein abundance among 18 samples from sheep and goats across a diverse range of fibers. We identified proteins with different abundance and are associated with variation in fiber features. Proteins with different abundance are mainly keratin or keratin-associated proteins (KRTAP11-1, KRT6A, KRT38), or are related to hair growth (DSC2, DSG3, EEF2, CALML5, TCHH, SELENBP1) and fatty acid synthesis (FABP4, FABP5). RNA-seq further confirmed the functional importance of the DSC2 gene in the determination of woolly phenotype in goat fibers. This comprehensive analysis of fibers from major fiber producing animals is the first to provide a list of candidate proteins that are involved in fiber formation. This list will be valuable asset for future studies into the molecular mechanisms that underlie fiber diversity.Biological significance: Proteins are the basis for animal-derived hair fibers, yet proteins conferring fiber structure and characteristics in sheep and goats are largely elusive. By examining 27 fibers samples representing 9 fiber types from sheep and goats through the iTRAQ approach, we show a list of differentially abundant proteins that are important to hair structural component, or genes related to hair growth and fatty acid synthesis. RNA-seq further validated the DSC2 gene is key to the woolly/straight hair phenotype in goats.