Transient Receptor Potential Melastatin 7 as an Independent Prognostic Factor in Human Esophageal Squamous Cell Carcinoma.
Transient Receptor Potential Melastatin 7 as an Independent Prognostic Factor in Human Esophageal Squamous Cell Carcinoma.
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DOI：
10.21873/anticanres.11429
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发表时间：
2017-03
影响因子：
2
通讯作者：
Shingo Nakashima;A. Shiozaki;D. Ichikawa;Shoichiro Hikami;T. Kosuga;H. Konishi;S. Komatsu;H. Fujiwara-H.-F
中科院分区：
文献类型：
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作者：
Shingo Nakashima;A. Shiozaki;D. Ichikawa;Shoichiro Hikami;T. Kosuga;H. Konishi;S. Komatsu;H. Fujiwara-H.-F
瞬时受体电位美拉他汀7 （TRPM7）是一种具有激酶活性的Mg2+/Ca2+可渗透通道，在许多实体肿瘤类型中表达。然而，尚未对食管鳞状细胞癌（ESCC）进行评估。本研究的目的是评估TRPM7在ESCC中的预后影响，并确定TRPM7在ESCC的增殖、迁移和侵袭中的作用。材料与方法对52例食管切除术ESCC患者原发肿瘤样本进行TRPM7免疫组化检测。实时聚合酶链反应（real-time polymerase chain reaction， PCR）分析了TRPM7在人ESCC细胞株中的表达。用TRPM7 siRNA进行敲低实验，分析其对细胞增殖、迁移和侵袭的影响。结果免疫组化染色显示，TRPM7存在于癌细胞细胞质中，其表达是术后良好生存的独立预后因素。用TRPM7- sirna抑制TRPM7的表达可提高TE5 ESCC细胞的增殖、迁移和侵袭能力。结论TRPM7的表达对ESCC患者的预后有影响。
BACKGROUND Transient receptor potential melastatin 7 (TRPM7) is a Mg2+/Ca2+-permeable channel with kinase activity and is expressed in many solid tumor types. However, it has not been evaluated in esophageal squamous cell carcinoma (ESCC). The aim of this study was to evaluate the prognostic impact of TRPM7 in ESCC and to determine the role of TRPM7 in proliferation, migration and invasion of ESCC. MATERIALS AND METHODS Immunohistochemistry of TRPM7 for 52 primary tumor samples obtained from patients with ESCC undergoing esophagectomy was performed. Expression of TRPM7 in human ESCC cell lines was analyzed by real-time polymerase chain reaction (PCR). Knockdown experiments were conducted with TRPM7 siRNA, and the effect on cell proliferation, migration and invasion was analyzed. RESULTS Immunohistochemical staining showed that TRPM7 was found in the cytoplasm of carcinoma cells, and that expression was an independent prognostic factor of good postoperative survival. Suppression of TRPM7 expression with TRPM7-siRNA increased the proliferation, migration and invasion of TE5 ESCC cells. CONCLUSION The expression of TRPM7 has an impact on the prognosis of patients with ESCC.