Functional variability of Bothrops atrox venoms from three distinct areas across the Brazilian Amazon and consequences for human envenomings
Functional variability of Bothrops atrox venoms from three distinct areas across the Brazilian Amazon and consequences for human envenomings
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DOI：
10.1016/j.toxicon.2019.04.001
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发表时间：
2019-06-01
期刊：
影响因子：
2.8
通讯作者：
Moura-da-Silva, Ana M.
中科院分区：
文献类型：
--
作者：
Del-Rei, Tiago H. Moretto;Sousa, Leijiane F.;Moura-da-Silva, Ana M.
蛇毒成分的变异性发生在不同的类群中，通常与蛇的适应性有关。这里，我们比较了B。研究人员对来自巴西亚马逊三个不同地理区域的atrox毒液进行了研究，并发现了显着的功能差异，特别是亚马逊河分隔的两个种群的毒液之间的差异，在Butantan serpentary研究所相同条件下出生，长大和保持的标本中。来自Presidente Figueiredo蛇的毒液诱导了更强的皮肤坏死，但对小鼠的促凝血和致死性较低;这些活动分别与毒液中PI类SVMP的存在和SVSP的不存在相关。来自圣本托蛇的毒液更具出血性，更有效地杀死小鼠，但诱导较低的皮肤坏死迹象，这与较高比例的SVMP和PI类SVMP亚型的缺乏相关。Belterra蛇，野生蛇的参考，提出毒液与中间表型。商业Bothrops抗蛇毒血清是有效的中和所有的生物活性，在这项研究中，包括皮肤坏死和促凝血，这是相关的人类蛇咬伤事故的B。阿托品与蛇适应性相关的功能差异也可能意味着B的不同适应性。阿托克斯蛇咬伤患者，值得临床毒理学家特别关注。
Variability in the composition of snake venoms occurs in different taxa and is usually correlated to snake fitness. Here, we compared B. atrox venoms from three different geographic regions across the Brazilian Amazon and found remarkable functional differences particularly between venoms from two populations separated by the Amazon River, in specimens born, raised and maintained under the same conditions at Institute Butantan serpentary. Venom from Presidente Figueiredo snakes induced stronger dermonecrosis, but was less procoagulant and lethal to mice; these activities were correlated to the presence of a PI-class SVMP and absence of a SVSP in the venom, respectively. Venom from Sao Bento snakes was more hemorrhagic, killed mice more efficiently, but induced lower signs of dermonecrosis, which was correlated to the higher proportion of SVMPs and the absence of a PI-class SVMP isoform. Belterra snakes, a reference of wild snakes, presented venoms with intermediate phenotypes. Commercial Bothrops antivenom was effective in neutralizing all biological activities evaluated in this study, including dermonecrosis and pro-coagulant, which are relevant for human snakebite accidents by B. atrox. Functional differences correlated to snake fitness may also imply in different symptomatology for B. atrox snakebite patients and deserve special attention from clinical toxicologists.