DNA vaccine encoding the Toxoplasma gondii bradyzoite-specific surface antigens SAG2CDX protect BALB/c mice against type II parasite infection.
DNA vaccine encoding the Toxoplasma gondii bradyzoite-specific surface antigens SAG2CDX protect BALB/c mice against type II parasite infection.
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DOI：
10.1016/j.vaccine.2013.07.065
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发表时间：
2013-09
期刊：
影响因子：
5.5
通讯作者：
Min Zhang-;Lingxiao Zhao;Jing Song;Y. Li;Qun-li Zhao;Shen-yi He;H. Cong
中科院分区：
文献类型：
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作者：
Min Zhang-;Lingxiao Zhao;Jing Song;Y. Li;Qun-li Zhao;Shen-yi He;H. Cong
弓形虫表面抗原SAG 2C、SAG 2D和SAG 2X在弓形虫缓殖子期特异性表达，对弓形虫包囊在脑内的持续存在具有重要意义。本研究以表达SAG 2C、SAG 2D和SAG 2X的T.并在BALB/c小鼠体内评价其保护效果。与PBS和pVAX 1处理的小鼠相比，用pVAX 1-SAG 2C（pSAG 2C）、pVAX 1 -2D（pSAG 2D）或pVAX 1 -2X（pSAG 2C）接种的小鼠显示出更高水平的血清IgG抗体和淋巴细胞增殖应答（p< 0.05）。免疫应答的特征在于强烈的Th 1应答和增加的IL-2和IFN-γ的细胞因子产生。免疫小鼠表现出显著的保护作用。PRU的gondii基因型II菌株（在小鼠中形成囊肿）。在单独用pSAG 2C（72%）、pSAG 2D（23%）、pSAG 2X（69%）免疫的小鼠中检测到脑囊肿负荷的显著降低，并且与PBS处理的小鼠相比，在组合组中实现了甚至更多的降低率77%。结果表明，表达SAG 2C、SAG 2D和SAG 2X的DNA疫苗，特别是这三种DNA质粒的组合免疫小鼠，可以有效地保护小鼠对抗T。生殖道慢性感染
The surface antigens SAG2C, SAG2D, and SAG2X, which expressed specifically on bradyzoite stage ofToxoplasma gondii, have been demonstrated to be important for persistence of cyst in the brain. In this study, DNA vaccines expressing SAG2C, SAG2D, and SAG2X ofT. gondiiwere constructed and their protective efficacy were evaluated in BALB/c mice. Mice vaccinated with pVAX1-SAG2C (pSAG2C), pVAX1-2D (pSAG2D) or pVAX1-2X (pSAG2C) showed higher levels of serum IgG antibodies and lymphocyte proliferation response compared to PBS and pVAX1 treated mice (p< 0.05). The immune response was characterized by a strong Th1 response and increased cytokine production of IL-2 and IFN-γ. Vaccinated mice displayed significant protection against the challenge with the cyst ofT. gondiigenotype II strain of PRU (cyst-forming in mouse). A significant reduction in the brain cyst burden was detected in the mice immunized with pSAG2C (72%), pSAG2D (23%), pSAG2X (69%) alone and even more reduction rate, 77%, was achieved in the combination group compared to PBS treated mice. The results implied that immunization with DNA vaccines expressing SAG2C, SAG2D, and SAG2X, and, in particular, a combination of all three DNA plasmids, could effectively protect the mice againstT. gondiichronic infection.