Behavior of Bioluminescent Transconjugants of Xanthomonas oryzae pv. oryzae in Compatible and Incompatible Rice Leaves
Behavior of Bioluminescent Transconjugants of Xanthomonas oryzae pv. oryzae in Compatible and Incompatible Rice Leaves
复制标题
米黄单胞菌生物发光转接合子的行为。
DOI：
10.3186/jjphytopath.65.93
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
O. Horino
中科院分区：
文献类型：
--
作者：
S. Tsuge;Y. Ikawa;Y. Hikichi;Y. Nakazawa;Kazumi Suzuki;Y. Kubo;O. Horino
转座子Tn 4431用于将荧光素酶（lux）操纵子引入黄单胞菌黄单胞菌致病型中。（X. O. PV. T7174。选择了两个具有高度生物发光性且与亲本菌株的致病性难以区分的接合子。在液体培养中，接合子在对数生长期产生更多的生物发光。从感染了接合子的水稻叶片中也检测到生物发光。在亲和水稻品种IR 24中，细菌在疾病症状表现之前繁殖和移动。与此相反，在不相容的水稻品种IR-BB 2，其中有抗性基因Xa 1和Xa 2，生物发光被限制在接种部位附近，但由transconjugants产生的光被检测到，甚至3周后接种，这表明细菌可以繁殖，但限制，并保持活力。没有细菌被重新分离出叶部分以外的光产生区域。生物发光的检测使得检查X射线的实际位置或感染过程成为可能。O. PV.不破坏受感染的叶片。
Transposon Tn4431 was used to introduce a luciferase (lux) operon into Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae) T7174. Two transconjugants which were highly bioluminescent and indistinguishable in pathogenicity with the parental strain were selected. The transconjugants produced more bioluminescence in the logarithmic phase in liquid cultures. Bioluminescence was also detected from rice leaves infected with the transconjugants. In the compatible rice cultivar IR24 bacteria multiplied and moved prior to the expression of disease symptoms. In contrast, in the incompatible rice cultivar IR-BB2, which has resistance genes Xa1 and Xa2, bioluminescence was restricted near the inoculation sites, but light produced by the transconjugants was detected even 3 weeks after inoculation, suggesting that bacteria could multiply, although restrictedly, and remain viable. No bacterium was reisolated from the leaf sections beyond the light-producing regions. The detection of bioluminescence makes it possible to examine the actual location or infection process of X. o. pv. oryzae continuously without destroying the infected leaves.