A rapid and convenient method for detecting a broad spectrum of malignant cells from malignant pleuroperitoneal effusion of patients using a multifunctional NIR heptamethine dye
A rapid and convenient method for detecting a broad spectrum of malignant cells from malignant pleuroperitoneal effusion of patients using a multifunctional NIR heptamethine dye
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使用多功能近红外七次甲基染料快速、便捷地检测患者恶性胸腹腔积液中的广谱恶性细胞
DOI：
10.1039/c4an01958j
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发表时间：
2015-01-01
期刊：
影响因子：
4.2
通讯作者：
Lu, Guangming
中科院分区：
文献类型：
--
作者：
Tian, Ying;Sun, Jing;Lu, Guangming
恶性胸腔积液中恶性细胞的检测对于制定或调整癌症患者的治疗方案至关重要。恶性胸腹腔积液的常规定性检测是细胞学检查，费时且复杂。因此，迫切需要一种更快速、更方便的检测策略。在这项研究中，我们报告了一种快速检测恶性胸腹水（胸水和腹水）的恶性细胞的患者使用IR-808，肿瘤靶向近红外（NIR）荧光七甲川染料（tNRI染料），它表现出上级标记效率没有特定的共轭生物标志物。通过与正常细胞的比较证实了IR-808对恶性细胞的靶向成像性能，并进行了IR-808在恶性积液中1 - 48 h的荧光稳定性测定。为了节省时间和剂量，将孵育时间和浓度优化为10 min和5 μM，用于检测28例恶性胸腹腔积液临床标本中的恶性细胞。结果表明，IR-808可被样品中的恶性细胞选择性地内化，并且这些恶性细胞在荧光显微镜下可容易地与正常细胞区分。细胞学检查与IR-808染色法的阳性率分别为86%（24/28）和79%（22/28）。两种方法之间具有良好的一致性水平（Kappa = 0.752，P < 0.001）。我们的研究结果表明，IR-808，一种新的近红外荧光七甲川染料，具有独特的光学成像和肿瘤靶向特性，可以提供一种快速，简单的方法来检测恶性胸腹腔积液患者的广谱恶性细胞。
Detection of malignant cells from malignant effusion is crucial to establish or adjust therapies of patients with cancer. The conventional qualitative detection in malignant pleuroperitoneal effusion is cytological analysis, which is time-consuming and complicated. Therefore, a faster and more convenient detection strategy is urgently needed. In this study, we report a rapid method to detect malignant cells from malignant pleuroperitoneal effusion (hydrothorax and ascites) of patients using IR-808, a tumor-targeted near-infrared (NIR) fluorescent heptamethine dye (tNRI dye), which exhibited superior labeling efficacy without specific conjugation to biomarkers. The targeted imaging performance toward malignant cells using IR-808 was confirmed by comparing with normal cells, and the fluorescence stability assay of IR-808 in malignant effusion was performed from 1 h to 48 h. In order to save time and dose, the incubation time and concentration were optimized to 10 min and 5 μM, which were used to detect malignant cells from 28 clinical samples of malignant pleuroperitoneal effusion. The results revealed that IR-808 could be internalized selectively by malignant cells of samples, and these malignant cells could be easily distinguished from normal cells under a fluorescence microscope. The positive rates between cytological analysis and the IR-808 staining method were 86% (24/28) and 79% (22/28), respectively. An excellent concordance level (Kappa = 0.752, P < 0.001) was observed between the two methods. Our results indicated that IR-808, a new NIR fluorescent heptamethine dye with unique optical imaging and tumor targeting properties, could provide a fast and simple way to detect a broad spectrum of malignant cells from malignant pleuroperitoneal effusion in patients.