Host-location kairomone fromPeriplaneta americana (L.) for parasitoidAprostocetus hagenowii (Ratzeburg)
Host-location kairomone fromPeriplaneta americana (L.) for parasitoidAprostocetus hagenowii (Ratzeburg)
复制标题
来自美洲大蠊 (L.) 的寄生蜂 Aprostocetus hagenowii (Ratzeburg) 的寄主位置利它素
DOI：
10.1007/bf02033575
复制
发表时间：
1996
影响因子：
2.3
通讯作者：
P. Koehler
中科院分区：
文献类型：
--
作者：
D. Suiter;D. Carlson;R. Patterson;P. Koehler
本文研究了姬小蜂Aprostocetus hagenowii（Ratzeburg）化学介导的寄主定位。在Y型管生物测定中，77.6%的雌蜂对美洲大蠊（Periplaneta americana（L.）卵鞘;寄生蜂没有响应空气没有挥发性刺激。成年蟑螂的排泄物就像卵囊一样诱人。生物测定的一个卵囊相当于五个脂质馏分（洗脱己烷和1，5，10，和30%的乙醚在己烷中）从硅胶柱色谱法表明，活性成分是烃。进一步用硝酸银浸渍硅胶柱层析法对卵鞘烃进行分离和生物活性测定，结果表明卵鞘烃的生物活性集中在一个组分中。该馏分的气相色谱（GC）分析显示出一个单峰;该峰在1963年、1969年和1972年被研究人员鉴定为（Z，Z）-6，9-二十七碳二烯。定性和定量的GC分析总烃从卵囊，粪便，和成年女性基本相同，6，9-heptacosadiene是占主导地位的烃从每个来源。粪中的链二烯含量是卵囊中的37倍。通过通气和Super Q吸附剂的吸附实验，研究了卵壳中二烯的挥发规律。目前的研究是（Z，Z）-6，9-heptacosadiene的生物活性的第一个证据，（Z，Z）-6，9-heptacosadiene是成年雌性美国蟑螂，其卵鞘和其粪便中的主要烃成分。
Chemically mediated host location in the eulophid parasitoidAprostocetus hagenowii(Ratzeburg) was investigated. In Y-tube bioassays 77.6% of female parasitoids responded to aPeriplaneta americana(L.) ootheca; parasitoids did not respond to air with no volatile stimuli. Frass from adult cockroaches was as attractive as an ootheca. Bioassay of one ootheca equivalent of five lipid fractions (eluted with hexane and 1, 5, 10, and 30% ether in hexane) from silica gel column chromatography indicated that the active component was a hydrocarbon. Further separation and bioassay of oothecal hydrocarbons by AgNO3-impregnated silica gel column chromatography indicated that the biological activity was in one fraction. Gas chromatographic (GC) analysis of this fraction revealed a single peak; this peak was identified by researchers in 1963, 1969, and 1972 as (Z,Z)-6,9-heptacosadiene. Qualitative and quantitative GC analyses of total hydrocarbons from oothecae, frass, and adult females were essentially identical; 6,9-heptacosadiene was the dominant hydrocarbon from each source. The alkadiene was 37 times more abundant in frass than on the ootheca. The volatilization of the alkadiene from oothecae was demonstrated by aeration and trapping on Super Q adsorbent. The current study is the first evidence for biological activity of (Z,Z)-6,9-heptacosadiene, a major hydrocarbon component on adult female American cockroaches, on their oothecae, and in their frass.