Cell growth and net Na+ flux are inhibited by a protein produced by kidney epithelial cells in culture.
Cell growth and net Na+ flux are inhibited by a protein produced by kidney epithelial cells in culture.
复制标题
细胞生长和净钠通量受到培养中肾上皮细胞产生的蛋白质的抑制。
DOI：
10.1073/pnas.81.3.793
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发表时间：
1984
影响因子：
11.1
通讯作者：
Holley,RW
中科院分区：
文献类型：
--
作者：
Walsh-Reitz,MM;Toback,FG;Holley,RW
汇合的肾上皮细胞培养物（BSC-1系）的增殖被细胞分泌的蛋白质（Mr约等于24，000）抑制。通过研究其对净Na+通量的影响来寻求这种生长抑制剂的作用机制，因为培养基中Na+的可用性增加已显示出刺激细胞生长。在纯化的抑制剂的存在下，在培养基更换后的生长刺激期间观察到的细胞Na+含量的增加被减弱。在蛋白质的存在下，细胞Na+积累和生长的抑制通过向培养基中加入NaCl而完全逆转。这些结果表明，净Na+通量和生长在肾上皮细胞的控制可以介导，至少部分，由分泌的细胞蛋白。
Proliferation of confluent kidney epithelial cell cultures (BSC-1 line) is inhibited by a protein (Mr approximately equal to 24,000) that is secreted by the cells. The mechanism of action of this growth inhibitor was sought by studying its effect on net Na+ flux because increased availability of Na+ in the culture medium had been shown to stimulate cell growth. The increase in cell Na+ content observed during stimulation of the growth after a medium change was attenuated in the presence of the purified inhibitor. Inhibition of both cell Na+ accumulation and growth in the presence of the protein was reversed completely by addition of NaCl to the medium. These results suggest that control of net Na+ flux and growth in kidney epithelial cells could be mediated, at least in part, by a secreted cellular protein.